Publication Date:
2000-11-18
Description:
PSD-95 is a neuronal PDZ protein that associates with receptors and cytoskeletal elements at synapses, but whose function is uncertain. We found that overexpression of PSD-95 in hippocampal neurons can drive maturation of glutamatergic synapses. PSD-95 expression enhanced postsynaptic clustering and activity of glutamate receptors. Postsynaptic expression of PSD-95 also enhanced maturation of the presynaptic terminal. These effects required synaptic clustering of PSD-95 but did not rely on its guanylate kinase domain. PSD-95 expression also increased the number and size of dendritic spines. These results demonstrate that PSD-95 can orchestrate synaptic development and are suggestive of roles for PSD-95 in synapse stabilization and plasticity.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉El-Husseini, A E -- Schnell, E -- Chetkovich, D M -- Nicoll, R A -- Bredt, D S -- New York, N.Y. -- Science. 2000 Nov 17;290(5495):1364-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Physiology, University of California, San Francisco 94143, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11082065" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Cells, Cultured
;
Dendrites/ultrastructure
;
Excitatory Postsynaptic Potentials
;
Hippocampus/cytology
;
Interneurons/cytology/metabolism/*physiology
;
Intracellular Signaling Peptides and Proteins
;
Membrane Proteins
;
Nerve Tissue Proteins/chemistry/genetics/metabolism/*physiology
;
Patch-Clamp Techniques
;
Presynaptic Terminals/physiology
;
Protein Structure, Tertiary
;
Pyramidal Cells/cytology/metabolism/*physiology
;
Rats
;
Receptor Aggregation
;
Receptors, AMPA/metabolism
;
Receptors, Glutamate/*metabolism
;
Receptors, N-Methyl-D-Aspartate/metabolism
;
Synapses/metabolism/*physiology
;
Synaptic Transmission
;
Synaptic Vesicles/physiology
;
Transfection
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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