Publication Date:
1989-06-16
Description:
Secretory chloride channels can be activated by adenosine 3',5'-monophosphate (cAMP)-dependent protein kinase in normal airway epithelial cells but not in cells from individuals with cystic fibrosis (CF). In excised, inside-out patches of apical membrane of normal human airway cells and airway cells from three patients with CF, the chloride channels exhibited a characteristic outwardly rectifying current-voltage relation and depolarization-induced activation. Channels from normal tissues were activated by both cAMP-dependent protein kinase and protein kinase C. However, chloride channels from CF patients could not be activated by either kinase. Thus, gating of normal epithelial chloride channels is regulated by both cAMP-dependent protein kinase and protein kinase C, and regulation by both kinases is defective in CF.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hwang, T C -- Lu, L -- Zeitlin, P L -- Gruenert, D C -- Huganir, R -- Guggino, W B -- 1-K08-HL02188/HL/NHLBI NIH HHS/ -- R01-DK 39619/DK/NIDDK NIH HHS/ -- R01-HL 40178/HL/NHLBI NIH HHS/ -- New York, N.Y. -- Science. 1989 Jun 16;244(4910):1351-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Physiology, Johns Hopkins University, Baltimore, MD 21205.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2472005" target="_blank"〉PubMed〈/a〉
Keywords:
Adult
;
Chloride Channels
;
Chlorides/*physiology
;
Cystic Fibrosis/*physiopathology
;
Electrophysiology
;
Fetus
;
Humans
;
In Vitro Techniques
;
Ion Channels/*physiology
;
Membrane Proteins/*physiology
;
Protein Kinase C/*physiology
;
Protein Kinases/*physiology
;
Respiratory System/cytology/physiopathology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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