Publication Date:
1989-01-13
Description:
The chemical synthesis of biologically active peptides and polypeptides can be achieved by using a convergent strategy of condensing protected peptide segments to form the desired molecule. An oxime support increases the ease with which intermediate protected peptides can be synthesized and makes this approach useful for the synthesis of peptides in which secondary structural elements have been redesigned. The extension of these methods to large peptides and proteins, for which folding of secondary structures into functional tertiary structures is critical, is discussed. Models of apolipoproteins, the homeo domain from the developmental protein encoded by the Antennapedia gene of Drosophila, a part of the Cro repressor, and the enzyme ribonuclease T1 and a structural analog have been synthesized with this method.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kaiser, E T -- Mihara, H -- Laforet, G A -- Kelly, J W -- Walters, L -- Findeis, M A -- Sasaki, T -- DK07825/DK/NIDDK NIH HHS/ -- GM12054/GM/NIGMS NIH HHS/ -- HL-186577/HL/NHLBI NIH HHS/ -- New York, N.Y. -- Science. 1989 Jan 13;243(4888):187-92.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Laboratory of Bioorganic Chemistry and Biochemistry, Rockefeller University, New York, NY 10021.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2492114" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Apolipoprotein A-I
;
Apolipoproteins A/chemical synthesis
;
Humans
;
Indicators and Reagents
;
Lipoproteins, HDL/chemical synthesis
;
Peptides/*chemical synthesis
;
Protein Conformation
;
Proteins/*chemical synthesis
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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