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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Journal of applied phycology 10 (1998), S. 31-36 
    ISSN: 1573-5176
    Keywords: Porphyra ; seaweed ; spectrophotometer ; triphenyltetrazolium ; viability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A spectrophotometric quantification method was optimized to evaluate its utility in seaweed tissue viability tests using the enzymatic reduction of colorless 2,3,5-triphenyltetrazolium chloride (TTC) to a colored triphenylformazan (TPF). To allow accurate determination of TPF in the seaweed Porphyra thallus and conchocelis, 0.2 g of tissues are incubated with 4 mL of 0.8% TTC reagent in the dark at 20°C for 1 h under a mineral oil layer. The TPF formed in tissues was extracted for 15 min at 60°C with 2 mL of 0.2 N KOH in 25% ethanol. Then TPF is partitioned away by prompt addition of hexane and vortexing. By this procedure, we have observed nearly complete separation of TPF, and observed good spectrophotometric discrimination between TPF and other hexane-soluble pigments at 545 nm. This procedure has proved applicable to a wide range of seaweed taxa; 1 species of Chlorophyta, 4 species of Phaeophyta and 7 species of Rhodophyta tested.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5176
    Keywords: compression ; LiCl ; Porphyra ; seaweed ; tension
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The mechanical properties of various differentiated regions of thePorphyra perforata thallus and the effect of LiCl were studied by performing compression and tension tests. Among the various differentiated tissues, the holdfast area was high in its ‘compressive modulus of elasticity’ and ‘tensile modulus of elasticity’, possibly related to its thick matrix. Vegetative non-dividing tissue and vegetative dividing tissue were the most flexible and strong, showing the highest ‘percentage elongation at break’ and ‘tensile strength’. The patch area, which is a transition zone leading to sexually mature tissue, had moderate values of tensile properties. Meanwhile, sexually differentiated male and female tissues had the highest ‘compressive modulus of elasticity’ and lowest tensile properties. Thes tisues tended to crumble easily. Treatments in LiCl, as used for DNA extraction, resulted in a decrease in both ‘compressive modulus of elasticity’ (87%) and ‘tensile modulus of elasticity’ (54%). After treatment of tissue for chromosome staining in a method using LiCl, there was a marked decrease in ‘tensile modulus of elasticity’ (49%), while the ‘compressive modulus of elasticity’ remained unchanged. Such mechanical changes verify the softening effect of LiCl on the seaweedP. perforata tissue.
    Type of Medium: Electronic Resource
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