Publication Date:
2002-01-05
Description:
Spermatogenesis is a precisely regulated process in which the germ cells closely interact with Sertoli cells. The molecular basis of this cell-cell adhesion is unknown. Here, we demonstrate that targeted disruption of Man2a2, a gene encoding alpha-mannosidase IIx (MX), an enzyme that forms intermediate asparagine-linked carbohydrates (N-glycans), results in Man2a2 null males that are largely infertile. The Man2a2 null spermatogenic cells fail to adhere to Sertoli cells and are prematurely released from the testis to epididymis. We identified an N-glycan structure that plays a key role in germ cell-Sertoli cell adhesion and showed that a specific carbohydrate was required for spermatogenesis.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Akama, Tomoya O -- Nakagawa, Hiroaki -- Sugihara, Kazuhiro -- Narisawa, Sonoko -- Ohyama, Chikara -- Nishimura, Shin-Ichiro -- O'Brien, Deborah A -- Moremen, Kelley W -- Millan, Jose Luis -- Fukuda, Michiko N -- CA 42595/CA/NCI NIH HHS/ -- CA71932/CA/NCI NIH HHS/ -- GM47533/GM/NIGMS NIH HHS/ -- HD05863/HD/NICHD NIH HHS/ -- RR05351/RR/NCRR NIH HHS/ -- U54 HD035041/HD/NICHD NIH HHS/ -- New York, N.Y. -- Science. 2002 Jan 4;295(5552):124-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Glycobiology Program and, Stem Cell Program, The Burnham Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11778047" target="_blank"〉PubMed〈/a〉
Keywords:
Acetylglucosamine/metabolism
;
Animals
;
Cell Adhesion
;
Cell Survival
;
Crosses, Genetic
;
Female
;
Gene Targeting
;
Glycopeptides/pharmacology
;
Infertility, Male/etiology
;
Lectins/metabolism
;
Male
;
Mannosidases/genetics/*metabolism
;
Mice
;
Mutation
;
Oligosaccharides/metabolism
;
*Plant Lectins
;
Polysaccharides/biosynthesis/chemistry/*metabolism
;
Sertoli Cells/*metabolism
;
Spermatocytes/metabolism/physiology
;
*Spermatogenesis
;
Spermatozoa/*metabolism/physiology
;
Testis/cytology/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
Permalink
