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Boundary analysis of sedimentation-velocity experiments with monodisperse and paucidisperse solutes (1978)

Van Holde, K. E. ; Weischet, Wolfgang O.

New York : Wiley-Blackwell

Biopolymers 17 (1978), S. 1387-1403

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ISSN: 0006-3525

Keywords: Chemistry ; Polymer and Materials Science

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Notes: A method is presented which allows one to calculate a distribution of sedimentation coefficients from the boundries of sedimentation-velocity experiments with mono- or pauci-disperse solutes. With two solutes differences in S value as small as 20% can be resolved. In absence of heterogeneity and concentration-dependent effects, the analysis also provides values for the diffusion coefficient within an accuracy of -10 to +5%. Tests with both simulated data and ultracentrifugation experiments on short DNA fragments show the value and the limitations of the method.

Additional Material: 10 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/bip.1978.360170602

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