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  • 1
    Electronic Resource
    Electronic Resource
    Quantitative analysis of the three regimes of DNA electrophoresis in agarose gels (1988)
    New York : Wiley-Blackwell
    Biopolymers 27 (1988), S. 509-524 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: An extensive series of experiments has been performed to study the mobility of DNA fragments ranging in size from 2.0 to 48.5 kilobose pairs. By varying the agarose concentration in the gels and the electric field strength, three DNA electrophoresis regimes were clearly identified: the Ogston regime (small DNA fragments in large pores of agarose), the reptation regime without DNA chain stretching (small pores of agarose and weak electric fields), and the reptation regime with DNA chain stretching (small pores of agarose, strong electric fields, and large DNA fragments). Here we report on the experimental identification of these regimes and on the conditions governing the transition between each of them. The onset of reptation and of stretching of DNA chains in gel electrophoresis are described quantitatively for the first time, and a phase diagram for the dynamics of DNA during electrophoresis is presented.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.360270311
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  • 2
    Electronic Resource
    Electronic Resource
    The biased reptation model of DNA gel electrophoresis: Mobility vs molecular size and gel concentration (1989)
    New York : Wiley-Blackwell
    Biopolymers 28 (1989), S. 1781-1791 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The biased reptation model provides a good framework for interpreting the results of continuous field DNA electrophoresis experiments performed in agarose gels. Here we discuss the main features of the mobility-molecular size and mobility-gel concentration diagrams as obtained from new extensive computer simulations of the model. Our aim is to suggest a global and coherent picture of this widely used yet poorly understood experimental technique, and to point out the areas where a systematic experimental study is still needed.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.360281011
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  • 3
    Electronic Resource
    Electronic Resource
    Prediction of chain elongation in the reptation theory of DNA gel electrophoresis (1985)
    New York : Wiley-Blackwell
    Biopolymers 24 (1985), S. 2181-2184 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.360241202
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  • 4
    Electronic Resource
    Electronic Resource
    On the stretching of DNA in the reptation theories of gel electrophoresis (1987)
    New York : Wiley-Blackwell
    Biopolymers 26 (1987), S. 863-872 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The reptation dynamics of DNA chains in gel electrophoresis is discussed. The differences between the Lumpkin, Déjardin, and Zimm (LDZ), and the Slater and Noolandi (SN), biased reptation models are pointed out and studied. We show that new assumptions are necessary in order to study large-field electrophoresis, and we present preliminary results of a computer simulation of DNA gel electrophoresis.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.360260607
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  • 5
    Electronic Resource
    Electronic Resource
    DNA gel electrophoresis: Effect of field intensity and agarose concentration on band inversion (1989)
    New York : Wiley-Blackwell
    Biopolymers 28 (1989), S. 1793-1799 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: We study the effect of electric field intensity and agarose gel concentration on the anomalous electrophoretic mobility recently predicted by the biased reptation model and experimentally observed for linear DNA fragments electrophoresed in continuous electric fields. We show that high fields and low agarose concentrations eliminate the physical mechanism responsible for anomalous DNA mobility and band inversion, in good agreement with theory, thus restoring the monotonic mobility-size relationship necessary for unambiguous interpretation of the results of DNA gel electrophoresis.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.360281012
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  • 6
    Electronic Resource
    Electronic Resource
    On the reptation theory of gel electrophoresis (1986)
    New York : Wiley-Blackwell
    Biopolymers 25 (1986), S. 431-454 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: We introduce a new biased reptation theory that allows a qualitative and semiquantitative study of DNA gel electrophoresis. We prove that stretching of the end-to-end vector of a very long charged reptating chain in an electric field occurs in a short period of time during a typical electrophoresis experiment, and that this leads to a field-dependent mobility only weaky dependent upon the size of the chain, in agreement with experiments on DNA. We also propose a practical technique to avoid chain stretching. Finally, many scaling laws are predicted, most of them being verifiable by experiment.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.360250305
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  • 7
    Electronic Resource
    Electronic Resource
    A computer simulation of trapping electrophoresis (1992)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part B: Polymer Physics 30 (1992), S. 1451-1457 
    Details
    ISSN: 0887-6266
    Keywords: gel electrophoresis ; sequencing ; reptation theory ; trapping electrophoresis ; computer simulation ; streptavidin ; biased reptation ; anomalous diffusion ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: The gel electrophoretic migration of streptavidin-DNA complexes is severely altered by the phenomenon known as “trapping electrophoresis.” We present a first computer simulation study of this process. Our simulations use the very efficient biased reptation algorithm. The steady state is characterized by a large increase in band broadening and interband separation. However, we also find that for a narrow range of molecular sizes, the separation power of gel electrophoresis is greatly increased. We discuss the implications of our findings for the possible improvement of DNA sequencing technologies. © 1992 John Wiley & Sons, Inc.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/polb.1992.090301304
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  • 8
    Electronic Resource
    Electronic Resource
    Construction of approximate entropic forces for finitely extensible nonlinear elastic (FENE) polymers (1994)
    Weinheim : Wiley-Blackwell
    Macromolecular Theory and Simulations 3 (1994), S. 695-704 
    Details
    ISSN: 1022-1344
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: When the stress applied to a Rouse-like polymer chain is large enough, one must use anharmonic entropic spring forces in order to keep the chain contour length from increasing to unphysical values. Although one can derive “exact” equations relating the spring extension to the entropic force produced by a finitely extensible non-linear elastic (FENE) random-walk polymer, such expressions are usually of little interest because their complexity would entail large evaluation times in numerical studies by computer. Moreover, these expressions can rarely be used directly in analytical studies. In this article, we describe a systematic method to construct analytically simple yet numerically accurate expressions to relate the entropic force to the extension of an entropic spring for a random-walk polymer chain in arbitrary dimension d ≥ 2. These expressions are modified Pade approximants which yield the correct asymptotic behaviours in both the small and large extension limits. It is shown that the well-known Warner empirical approximation is but a limiting case (for infinite dimensions).
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mats.1994.040030405
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  • 9
    Electronic Resource
    Electronic Resource
    Generalized rouse model for polymer melt dynamics (1987)
    Basel : Wiley-Blackwell
    Die Makromolekulare Chemie, Rapid Communications 8 (1987), S. 51-58 
    Details
    ISSN: 0173-2803
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/marc.1987.030080110
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