Publication Date:
2022-05-25
Description:
Author Posting. EHP is a publication of the United States government. Publication of EHP lies in the public domain and is therefore without copyright. The definitive version was published in Environmental Health Perspectives 112 (2004): 511-515, doi:10.1289/ehp.6719.
Description:
Structural differences were identified in gill DNA from two groups of English sole collected from
Puget Sound, Washington, in October 2000. One group was from the industrialized Duwamish
River (DR) in Seattle and the other from relatively clean Quartermaster Harbor (QMH).
Chemical markers of sediment contamination [e.g., polynuclear aromatic hydrocarbons (PAHs)
and polychlorinated biphenyls (PCBs)] established that the DR was substantially more contaminated
than QMH. The levels of these chemicals in the sediments of both sites were consistent with
levels of cytochrome P450 1A (CYP1A) expression in the gills of English sole from the same sites.
Structural differences in gill DNA between the groups were evinced via statistical models of Fourier
transform-infrared (FT-IR) spectra. Marked structural damage was found in the gill DNA of the
DR fish as reflected in differences in base functional groups (e.g., C–O and NH2) and conformational
properties (e.g., arising from perturbations in vertical base stacking interactions). These
DNA differences were used to discriminate between the two fish groups through principal components
analysis of mean FT-IR spectra. In addition, logistic regression analysis allowed for the development
of a “DNA damage index” to assess the effects of contaminants on the gill. The evidence
implies that environmental chemicals contribute to the DNA changes in the gill. The damaged
DNA is a promising marker for identifying, through gill biopsies, contaminant effects on fish.
Description:
This publication was made possible by grant P42
ES04696 (D.C.M.) and 5-P42-ESO7381 (J.J.S.)
from the National Institute of Environmental Health
Sciences (NIEHS), National Institutes of Health
(NIH).
Keywords:
Polynuclear aromatic hydrocarbons (PAH)
;
Polychlorinated biphenyls (PCBs)
;
DNA markers
;
Contaminant effects,
;
Cytochrome P450 1A
;
DNA structure
;
Fish gills
;
Fourier transform-infrared spectroscopy
Repository Name:
Woods Hole Open Access Server
Type:
Article
Format:
519655 bytes
Format:
application/pdf
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