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  • 1
    Electronic Resource
    Electronic Resource
    Detection of 5S rDNA and other repeated DNA on supernumerary B chromosomes ofTriticum species (Poaceae) (1995)
    Springer
    Plant systematics and evolution 196 (1995), S. 131-139 
    Details
    ISSN: 1615-6110
    Keywords: Poaceae ; Triticum speltoides ; T. tripsacoides ; Supernumerary B chromosomes ; C-banding ; in situ hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The supernumerary B chromosomes ofTriticum speltoides andT. tripsacoides were analyzed in mitotic metaphases of spike primordium cells by C-banding and in situ hybridization (ISH) analyzes. B chromosomes ofT. speltoides have larger telomeric and interstitial C-bands, whereas those ofT. tripsacoides are almost completely devoid of C-bands. A prominent ISH site of rye related DNA sequences (using probe pSc119) was detected on B chromosomes ofT. tripsacoides and only a minor ISH site was observed on theT. speltoides B chromosomes. However, two ISH sites of 5S rRNA loci were detected at a terminal and an interstitial location of theT. speltoides B chromosomes. These sites were absent on B chromosomes ofT. tripsacoides. The results are discussed with respect to the phylogenetic origin of these B chromosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00982954
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  • 2
    Electronic Resource
    Electronic Resource
    Intraspecific karyotype divergence inTriticum araraticum (Poaceae) (1994)
    Springer
    Plant systematics and evolution 192 (1994), S. 117-145 
    Details
    ISSN: 1615-6110
    Keywords: Poaceae ; Triticum araraticum ; Karyotype ; C-banding polymorphism ; chromosomal aberrations ; intraspecific divergence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Karyotypes of 185 accessions ofTriticum araraticum Jakubz. (2n = 28 = 4x = AtAtGG) from Iraq, Iran, Turkey, and Transcaucasia were analyzed using C-banding technique. All accessions showed a certain degree of C-banding polymorphism and further karyotypic diversity was generated by structural rearrangements, mainly translocations. Eighty-one accessions had the normal karyotype similar to that ofT. timopheevii (cultivation), i.e., they showed C-banding polymorphism but no chromosomal rearrangements based on the resolving power of the C-banding technique. One-hundred four accessions showed 34 karyotypic variants, 31 had reciprocal translocations with the breakpoints in the centromeric regions of chromosomes. Three showed reciprocal translocations with the breakpoints in intercalary regions of chromosomes. A paracentric inversion for 7At chromosome was observed in some accessions. The rearranged karyotypes differed from the normal by one translocation in 21 variants, by two in 9 variants, by three in 1 variant, and by four in 2 variants of karyotypes. Translocations occurred more frequenty in the chromosomes of G-genome than of At-genome. Individual chromosomes differed in the frequencies of their involvement in translocations. Each geographical region contained a unique spectrum of translocations. Karyotypic diversity was the highest in Iraq followed by Transcaucasia and Turkey. Iran showed little karyotypic variation. Based on karyotypic analysis, Iraq should be considered as a centre of origin and primary centre of diversity ofT. araraticum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00985912
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  • 3
    Electronic Resource
    Electronic Resource
    Standard karyotypes ofAegilops uniaristata, Ae. mutica, Ae. comosa subspeciescomosa andheldreichii (Poaceae) (1996)
    Springer
    Plant systematics and evolution 202 (1996), S. 199-210 
    Details
    ISSN: 1615-6110
    Keywords: Poaceae ; Aegilops uniaristata ; Ae. mutica ; Ae. comosa subsp.comosa ; Ae. comosa subsp.heldreichii ; C-banding ; karyotypes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract C-banding patterns and polymorphisms were analyzed in several accessions of the diploidAegilops speciesAe. uniaristata, Ae. mutica, andAe. comosa subsp.comosa and subsp.heldreichii, and standard karyotypes of these species were established. Variation in C-band size and location was observed between different accessions, but did not prevent chromosome identification. One accession ofAe. uniaristata was homozygous for whole-arm translocations involving chromosomes 1N and 5N. The homoeologous relationships of these chromosomes were established by comparison of chromosome morphologies and C-banding patterns to other diploidAegilops species with known chromosome homoeology. In addition, in situ hybridization analysis with a 5S rDNA probe was used to identify homoeologous groups 1 and 5 chromosomes. The present analysis permitted the assignment of allAe. mutica, comosa subsp.comosa, andAe. comosa subsp.heldreichii chromosomes, and three of the sevenAe. uniaristata chromosomes according to their homoeologous groups. The data presented will be useful analyzing genome differentiation in polyploidAegilops species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00983382
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  • 4
    Electronic Resource
    Electronic Resource
    Relationships betweenNor-loci from differentTriticeae species (1988)
    Springer
    Plant systematics and evolution 160 (1988), S. 77-89 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Poaceae ; Triticeae ; Triticum aestivum ; T. timopheevi ; T. monococcum ; Dasypyrum villosum ; B, G, S, A, V genomes ; Nor-loci ; DNA-DNA hybridization ; spacer rDNA sequences ; molecular evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract TheNor-loci of polyploid wheats and their putative diploid progenitor species were assayed by probing isolated nuclear DNA with ribosomal DNA spacer sequences (spacer rDNA sequences, isolated by cloning), from theNor-loci of genomes B (Triticum aestivum), G (T. timopheevi), B (syn. S,T. speltoides), A (T. monococcum) and V (Dasypyrum villosum). DNA samples for analysis were digested with the restriction endonuclease Taq 1 and assayed by DNA-DNA hybridization under standard (37°C) and high stringency (64°C) conditions. The assay procedure emphasized differences between the divergent spacer sequences of the polyploid species and allowed relative homologies to the respective sequences in diploid species to be established. — The studies indicated thatT. timopheevi andT. speltoides contain different sets of spacer rDNA sequences which were readily distinguishable and, in the case ofT. timopheevi, assigned toNor-loci on different chromosomes. This contrast with the spacer rDNA sequences of the majorNor-loci on chromosomes 1 B and 6 B inT. aestivum, which were difficult to distinguish and were deduced to contain very similar sequences. Among the diploid progenitor species only the spacer rDNA fromT. speltoides shared close homology with polyploid wheat species. OneNor-locus inT. timopheevi (on chromosome 6 G) did not show close homology with any of the rDNA spacer probes available. — The data suggestsT. speltoides was the origin of someNor-loci for both theT. timopheevi andT. turgidum lines of tetraploid wheats. The possibility that the 6GNor-locus inT. timopheevi may have derived from an unknown diploid species by introgressive hybridization is discussed. The spacer rDNA sequence probe fromT. monococcum shared good homology with some accessions ofD. villosum and a line ofT. dicoccoides; the implications of this finding for evolution of present-day wheats are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00936711
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  • 5
    Electronic Resource
    Electronic Resource
    Frequent duplication and deletion events in the 5S RNA genes and the associated spacer regions of theTriticeae (1988)
    Springer
    Plant systematics and evolution 160 (1988), S. 105-122 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Poaceae ; Triticeae ; 5 S DNA sequence analyses ; duplication and deletion events ; phenetic relationships
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The 5 S DNA units from 15 grasses in theTriticeae were analysed at the DNA sequence level. Four units carried duplications near the 3′-end of the 5 S RNA gene with 3 of the duplications centred on the same base pairs as a duplication previously reported byGerlach & Dyer. The fourth duplication was located 3′ downstream from the gene, in the spacer region. Apparent deletions were very frequent when units of the different grasses were compared and it was clear that these deletions did not extend into a 75 bp spacer region upstream from the 5 S RNA gene. This 75 bp region also tended to be more conserved between the grasses as compared to the high level of sequence change in the rest of the spacer region. — Phenetic relationships were established between the grasses using the sequence data. The relationships were generally consistent with the data from other parameters and, in addition, showed that two Australian grasses were closely related to the other Northern hemisphere genera examined. The data concerning the Australian grasses is discussed in relation to the isolated nature of Australia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00936713
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  • 6
    Electronic Resource
    Electronic Resource
    Fractionation of alcohol dehydrogenase and α-amylase alleles from wheat in actinomycin-D/CsCl gradients (1988)
    Springer
    Plant systematics and evolution 160 (1988), S. 61-64 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Poaceae ; Triticum ; Fractionation of alcohol dehydrogenase and α-amylase ; actinomycin-D/CsCl gradient ; cloning of alleles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Wheat alcohol dehydrogenase and α-amylase genes were fractionated and enriched in an actinomycin-D/CsCl gradient. The experiments illustrated may be of importance for cloning of DNA alleles in crop science.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00936709
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