ISSN:
1432-2242
Keywords:
Nicotiana
;
Somatic hybrids
;
Streptomycin resistance
;
Plastid segregation
;
Plastid DNA
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Protoplasts of Nicotiana tabacum SRI (streptomycin resistant) and of Nicotiana knightiana (streptomycin sensitive) were fused using polyethylene glycol treatment. From three heterokaryons 500 clones were obtained. From the 43 which were further investigated, 6 resistant, 3 sensitive, and 34 chimeric (consisting of resistant and sensitive sectors) calli were found. From eight clones, a total of 39 plants were regenerated and identified as somatic hybrids. Chloroplast type (N. tabacum = NT or N. knightiana = NK) in the plants was determined on the basis of the species specific EcoRI restriction pattern of the chloroplast DNA. Regenerates contained NT (13 plants) or NK (15 plants) plastids but only the plants with the NT chloroplasts were resistant to streptomycin. This finding and our earlier data on uniparental inheritance points to the chloroplasts as the carriers of the streptomycin resistance factor.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00264975
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