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Asymmetric somatic hybrids between Lycopersicon esculentum and irradiated Lycopersicon peruvianum (1990)

Wijbrandi, J. ; Posthuma, A. ; Kok, J. M. ; [et al.]

Springer

Theoretical and applied genetics 80 (1990), S. 305-312

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ISSN: 1432-2242

Keywords: Protoplast fusion ; Gamma irradiation ; Plant regeneration ; Aneuploidy ; Partial genome transfer

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary Asymmetric somatic hybrids of Lycopersicon esculentum and Lycopersicon peruvianum were obtained by fusion of leaf protoplasts from both species after irradiation of protoplasts or leaf tissue of L. peruvianum with 50, 300, or 1,000 Gy of gamma-rays. These radiation doses were sufficient to abolish the growth of the L. peruvianum protoplasts. The hybrids were selected for their ability to regenerate plants; this regeneration capacity derived from L. peruvianum. All asymmetric hybrid plants were aneuploid. The ploidy level, the morphology, and the regeneration rate were analyzed in relation to the radiation dose applied to L. peruvianum. After a low dose (50 Gy), most hybrids had near-triploid chromosome numbers, whereas after a high dose (300 or 1,000 Gy), most hybrids had near-pentaploid numbers. The morphology of the asymmetric hybrids was intermediate between that of L. esculentum and symmetric somatic hybrids of both species (obtained without irradiation treatment), and approached the morphology of L. esculentum to a greater extent after a high dose of irradiation. The asymmetric hybrids regenerated more slowly than the symmetric hybrids and regeneration proceeded more slowly after a high dose than after a low dose of irradiation. The high-dose hybrids also grew more slowly, flowered less, and set fruits less than the low-dose hybrids. No seeds could be obtained from any asymmetric hybrid.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00210064

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The effect of the nature of the polymer backbone on the stability and the analytical response of polymer-modified electrodes (1995)

Doherty, A. P. ; Stanley, M. A. ; Arana, G. ; [et al.]

Weinheim : Wiley-Blackwell

Electroanalysis 7 (1995), S. 333-339

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ISSN: 1040-0397

Keywords: Modified electrode ; Redox polymer ; Sensor ; Flow injection analysis ; Iron ; Chemistry ; Polymer and Materials Science

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Notes: The synthesis, characterization, and sensor application of the novel redox polymer [Os(bipy)2(PS)7.5(DMAP)2.5Cl]Cl, where bipy = 2,2′-bipyridyl, PS = polystyrene, and DMAP = poly[4-(N-methyl-N-p-vinylbenzylamino) pyridine], are described. The charge transport properties of electrodes modified with the redox material are investigated using cyclic voltammetry and chronoamperometry. The modified electrode behaves as an efficient electrocatalyst for the outer-sphere reduction of FeIII, with the cross-exchange reaction occurring at the surface of the polymer (Sk″) at concentrations less than 1.0 × 10-3 mol dm-3 FeIII, with a change over to the Ste, kinetic regime at higher substrate concentrations. Direct agreement was observed between the kinetic behavior at rotating disk electrodes and in thin-layer flow cells. Application of the modified electrodes for the determination of iron in pharmaceutical formulations and the long term stability of the electrodes are investigated. The results obtained are compared with those reported for the analogous metallopolymer [Os(bipy)2(PVP)10Cl]Cl, where PVP is poly-4-vinylpyridine.

Additional Material: 7 Ill.