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  • Plant regeneration  (1)
  • Scanning tunneling microscope  (1)
  • TCDD  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Plant regeneration from in vitro-cultured seedling leaf protoplasts of Actinidia eriantha Benth (1998)
    Springer
    Plant cell reports 17 (1998), S. 819-821 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsActinidia eriantha ; Chromosome number ; Multinucleate cells ; Plant regeneration ; Protoplast culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Newly expanded in vitro leaves of Actinidia eriantha were used for protoplast isolation. Protoplasts were cultured in liquid MS medium (lacking NH4NO3) supplemented with 2,4-D (2,4-dichlorophenoxyacetic acid) and 0.4 M glucose. The plating efficiency after 3 weeks of culture was 19.4%, and calli were recovered without addition of fresh medium. These calli regenerated shoots on transfer to MS medium containing 2.28 μM zeatin and 0.57 μM IAA (indole-3-acetic acid). Regenerated shoots were rooted by immersion in 20 ppm IBA (indole-3-butyric acid) solution before culturing on half-strength MS medium lacking growth regulators. Somaclonal variation, in terms of chromosome number and nuclei per cell of protoplast-derived plants, was estimated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050490
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  • 2
    Electronic Resource
    Electronic Resource
    Scanning tunneling microscopy of laser-deposited YBa2Cu3O7 thin films on Y-stabilized zirconia substrate (1995)
    Springer
    Journal of superconductivity 8 (1995), S. 103-108 
    Details
    ISSN: 1572-9605
    Keywords: Scanning tunneling microscope ; high-temperature superconducting thin film ; growth mechanism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Physics
    Notes: Abstract The surface morphology and growth mechanism ofc-oriented YBa2Cu3O7 thin films deposited on Y-stabilized zirconia substrate by laser deposition has been investigated with scanning tunneling microscopy. Both screw dislocation and layered island growth processes took place. Surface features such as screw dislocation, steps, subgrain boundaries, holes, and troughs were present, suggesting that they may act as pinning sites for flux lines. A thin layer of nonsuperconducting material with a thickness of a few angstroms is seen on the surface of the film.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00732249
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  • 3
    Electronic Resource
    Electronic Resource
    Derivation and characterization of a zebrafish liver cell line (1994)
    Springer
    Cell biology and toxicology 10 (1994), S. 167-176 
    Details
    ISSN: 1573-6822
    Keywords: cytochrome P450 ; liver cell culture ; TCDD ; zebrafish
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract ZF-L cells were derived from normal adult zebrafish liver, and have been growing in culture for more than 100 generations. The cells were derived in basal nutrient medium supplemented with fetal bovine serum (FBS), trout serum, trout embryo extract, bovine insulin and mouse epidermal growth factor. After 50 generations in culture, optimal growth of the cells was achieved in medium supplemented with FBS (5%) and trout serum (0.5%). ZF-L cells were hypodiploid (modal chromosome number= 46) and exhibited an epithelial morphology. ZF-L cell homogenates exhibited alanine and aspartate aminotransferase, glucose-6-phosphatase and alkaline phosphatase enzyme activities. The cells synthesized and released several proteins into the culture medium, including a 70 kDa protein recognized by anti-bovine serum albumin IgG.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00757560
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