ISSN:
1432-2242
Keywords:
Key words mtDNA
;
Polymorphism
;
Pinus ponderosa
;
Pinus flexilis
;
Pinus sylvestris
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The b/c intron of the mitochondrial nad1 gene, was sequenced to characterize the indel region of ponderosa pine, Pinus ponderosa. The sequence in ponderosa pine was aligned with the sequence in Scots pine, Pinus sylvestris, to design seven primers that are useful for sequencing and for revealing size variation in amplified fragments in ponderosa pine, Scots pine, and limber pine, Pinus flexilis. These primers reveal variability in all three species, and the pattern of variability within ponderosa pine is described by a preliminary survey. The indel region of ponderosa pine contains three distinct elements with lengths of 31, 32, and 34 bp.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s001220051606
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