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  • 1
    Publication Date: 2021-05-19
    Description: A study was carried out to evaluate the probiotic activity of Bacillus subtilis G1 isolated from fermented pickles in growth performance and disease resistance of Hemibagrus nemurus fingerlings at Universiti Putra Malaysia. The probiotic was mixed in feed at doses of 0 (C, control), 3 x 109 (T1) 3 x 107 (T2) and 3 x 105 (T3) cfu g-1 and fed to the catfish fingerlings for nine weeks. Results showed that catfish fed a diet containing 107 cfu g-1 B. subtilis G1 had significantly higher percent weight gain (248.69 ± 3.31%), and better food conversion ratio (1.68 ± 0.03), than those of other treatments. Inhibitory activity of the probiotic B. subtilis G1 against fish pathogens Aeromonas hydrophila and Streptococcus agalactiae was evaluated by well diffusion agar method. Inhibition zones measured showed A. hydrophila and S. agalactiae were 16.13 ± 0.91 mm and 17.5 ± 1.84 mm, respectively, indicating strong inhibitory activity against the pathogens. Three weeks after the feeding trial, the fingerlings were challenged with 0.1 ml containing 106 cfu ml-1 of A. hydrophila by intra-peritoneal injection. After 14 days, the mortality rate of catfish was significantly lower in group T1 (30 ± 5.8%) compared to the control (C) group (56.7 ± 3.3%). The findings of this study proved that administration of B. subtilis G1 can improve growth and disease resistance in catfish.
    Description: Published
    Keywords: Biology ; Physiology ; Hemibagrus nemurus ; Probiotic ; Growth performance ; Disease resistance ; Aeromonas hydrophila ; Feeding ; Fingerlings
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.846-856
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  • 2
    Publication Date: 2021-05-19
    Description: This study was conducted to determine the optimal dietary protein requirement for lemon fin barb hybrid fingerlings. Triplicate groups of fish (1.00 ± 0.05 g) were fed twice a day until apparent satiation with five isocaloric (16 kJ/g) diets containing varying protein level ranging from 20 to 40% for 60 days. Survival was not affected by the dietary protein level. The weight gain and specific growth rate were improved with dietary protein level up to 35 %. The best feed conversion ratio (1.61) was achieved at 35% dietary protein level. Protein efficiency ratio was not influenced by the dietary protein level. Using a broken line regression model, the dietary protein requirement of lemon fin barb hybrid was estimated at 34.6 % based on weight gain response but the value was not significantly better than the 30% protein diet. Proximate composition of the fish showed that dietary protein level only affected the protein content of the lemon fin barb hybrid. The study showed the protein retention was increased until 30% and decreased above this level. Lipid retention decreased as the dietary protein increased. It can be concluded that lemon fin barb hybrid required 30-35% protein at gross energy 16 kJ/g for the best growth and feed efficiency.
    Description: Published
    Keywords: Biology ; Physiology ; Lemon fin barb hybrid ; Lemon fin barb hybrid ; Protein requirement ; Diet ; Growth ; Nutrition ; Body composition ; Feed ; Fingerlings
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.425-435
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  • 3
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    In:  http://aquaticcommons.org/id/eprint/22821 | 18721 | 2018-05-24 22:41:21 | 22821 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-10
    Description: A study was carried out to evaluate the probiotic activity of Bacillus subtilis G1 isolated from fermented pickles in growth performance and disease resistance of Hemibagrus nemurus fingerlings at Universiti Putra Malaysia. The probiotic was mixed in feed at doses of 0 (C, control), 3 x 10^9 (T1) 3 x 10^7 (T2) and 3 x 10^5 (T3) cfu g^-1 and fed to the catfish fingerlings for nine weeks. Results showed that catfish fed a diet containing 10^7 cfu g^-1 B. subtilis G1 had significantly higher percent weight gain (248.69 ± 3.31%), and better food conversion ratio (1.68 ± 0.03), than those of other treatments. Inhibitory activity of the probiotic B. subtilis G1 against fish pathogens Aeromonas hydrophila and Streptococcus agalactiae was evaluated by well diffusion agar method. Inhibition zones measured showed A. hydrophila and S. agalactiae were 16.13 ± 0.91 mm and 17.5 ± 1.84 mm, respectively, indicating strong inhibitory activity against the pathogens. Three weeks after the feeding trial, the fingerlings were challenged with 0.1 ml containing 10^6 cfu ml^-1 of A. hydrophila by intra-peritoneal injection. After 14 days, the mortality rate of catfish was significantly lower in group T1 (30 ± 5.8%) compared to the control (C) group (56.7 ± 3.3%). The findings of this study proved that administration of B. subtilis G1 can improve growth and disease resistance in catfish.
    Keywords: Aquaculture ; Biology ; Fisheries ; Hemibagrus nemurus ; Probiotic ; Growth performance ; Disease resistance ; Aeromonas hydrophila ; Biology ; Physiology ; Malaysia
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 846-856
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  • 4
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    Unknown
    In:  http://aquaticcommons.org/id/eprint/22786 | 18721 | 2018-05-22 18:11:10 | 22786 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-10
    Description: This study was conducted to determine the optimal dietary protein requirement for lemon fin barb hybrid fingerlings. Triplicate groups of fish (1.00 ± 0.05 g) were fed twice a day until apparent satiation with five isocaloric (16 kJ/g) diets containing varying protein level ranging from 20 to 40% for 60 days. Survival was not affected by the dietary protein level. The weight gain and specific growth rate were improved with dietary protein level up to 35 %. The best feed conversion ratio (1.61) was achieved at 35% dietary protein level. Protein efficiency ratio was not influenced by the dietary protein level. Using a broken line regression model, the dietary protein requirement of lemon fin barb hybrid was estimated at 34.6 % based on weight gain response but the value was not significantly better than the 30% protein diet. Proximate composition of the fish showed that dietary protein level only affected the protein content of the lemon fin barb hybrid. The study showed the protein retention was increased until 30% and decreased above this level. Lipid retention decreased as the dietary protein increased. It can be concluded that lemon fin barb hybrid required 30-35% protein at gross energy 16 kJ/g for the best growth and feed efficiency.
    Keywords: Aquaculture ; Biology ; Fisheries ; Lemon fin barb hybrid ; protein requirement ; diet ; growth ; nutrition ; body composition ; Biology ; Physiology ; Malaysia
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 425-435
    Location Call Number Expected Availability
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