ISSN:
1617-4623
Keywords:
C4 photosynthesis
;
cDNA probes
;
Photosynthetic carbon reduction cycle enzymes
;
Restriction fragment length polymorphism analysis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Homologous probes for the wheat coding sequences of the enzymes phosphoribulokinase, phosphoglycerate kinase (both chloroplast and cytosolic forms), chloroplast fructose-1,6-bisphosphatase and the small subunit of ribulose-1,5-bisphosphate carboxylase were used to determine the copy number and chromosomal location of the genes encoding these enzymes by restriction fragment length polymorphism analysis. Heterologous probes were similarly used to characterize the genes for the enzymes glyceraldehyde phosphate dehydrogenase (both chloroplast and cytosolic forms), phosphoenolpyruvate carboxylase and pyruvate, orthophosphate dikinase. Several of the genes are present in single copies per haploid genome, and the different enzymes are encoded by loci dispersed on different chromosomes. The significance of these findings is discussed in relation to gene expression and control of copy number.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00332405
Permalink
