Publication Date:
2010-05-08
Description:
In neurons, synaptotagmin 1 (Syt1) is thought to mediate the fusion of synaptic vesicles with the plasma membrane when presynaptic Ca2+ levels rise. However, in vitro reconstitution experiments have failed to recapitulate key characteristics of Ca2+-triggered membrane fusion. Using an in vitro single-vesicle fusion assay, we found that membrane-anchored Syt1 enhanced Ca2+ sensitivity and fusion speed. This stimulatory activity of membrane-anchored Syt1 dropped as the Ca2+ level rose beyond physiological levels. Thus, Syt1 requires the membrane anchor to stimulate vesicle fusion at physiological Ca2+ levels and may function as a dynamic presynaptic Ca2+ sensor to control the probability of neurotransmitter release.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2994549/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2994549/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lee, Han-Ki -- Yang, Yoosoo -- Su, Zengliu -- Hyeon, Changbong -- Lee, Tae-Sun -- Lee, Hong-Won -- Kweon, Dae-Hyuk -- Shin, Yeon-Kyun -- Yoon, Tae-Young -- R01 GM051290/GM/NIGMS NIH HHS/ -- R01 GM051290-16/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2010 May 7;328(5979):760-3. doi: 10.1126/science.1187722.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Physics, KAIST, Daejeon 305-701, South Korea.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/20448186" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Calcium/*metabolism
;
Magnesium/metabolism
;
*Membrane Fusion
;
Membrane Lipids/metabolism
;
Neurotransmitter Agents/metabolism
;
Phosphatidylinositol 4,5-Diphosphate/metabolism
;
Rats
;
SNARE Proteins/metabolism
;
Synaptic Vesicles/*physiology
;
Synaptotagmin I/chemistry/*metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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