ISSN:
1432-2048
Keywords:
Carbohydrate antigens (alginate, fucan)
;
Cell wall
;
Immunofluorescence
;
Fucus
;
Monoclonal antibody
;
Phaeophyta
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Monoclonal antibodies to cell wall carbohydrates were produced against carbohydrates extracted from the brown alga, Fucus distichus ssp. edentatus (de la Pyl.) Powell. Mouse spleen cells were immunized in vitro with alginate and fucans, and hybridoma cultures were screened by enzyme immunoassay. Most antibodies were immunoglobulin (Ig)M and one was IgA. Antigens were localized on methacrylate sections of Fucus tissues by indirect immunofluorescence. Each antibody labelled tissues with a distinctive distribution pattern in cell walls and extracellular matrix regions, demonstrating that each antibody was specific for a different extracellular epitope (i.e., antigenic determinant). Most antibodies also labelled intracellularly on at least one cell type. Punctate, fibrous or clumped labelling was characteristic of individual antibodies and provided information related to carbohydrate structure and solubility. These antibodies are molecular probes for small regions on cell wall polymers and should be valuable in studies of cell wall synthesis, secretion, assembly and modification as well as carbohydrate fine structure and function.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00399916
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