ISSN:
1432-0983
Keywords:
Overexpression
;
Peroxisomes
;
Saccharomyces cerevisiae
;
Stabilization
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We have constructed a gene coding for the 12-kDa intermediate form of the 2s methionine-rich protein from Bertholletia excelsa seeds. This protein, expressed intracellularly in yeast, is characterised by a 20-min balf-life. By adding 11 amino acids corresponding to the peroxisome-targeting sequence (PTSc) of luciferase, we have significantly increased its half-life. This stabilization allowed accumulation of the BZN protein into the peroxisome as judged by cell fractionation. Accumulation of the 12-kDa protein results in a significant increase of the total methionine content in yeast cells (30%) indicating that such a microorganism could represent a practicable protected shuttl for an animal-feed additive.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00309924
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