Publication Date:
1991-04-19
Description:
Evidence indicates that cytotoxic T lymphocytes (CTLs) may be important in containing the spread of the human immunodeficiency virus (HIV) in the infected host. Although the use of recombinant viruses has been proposed as an approach to elicit protective immunity against HIV, the ability of recombinant viral constructs to elicit CD8+ CTL responses in higher primates has never been demonstrated. A live recombinant virus, vaccinia-simian immunodeficiency virus of macaques (SIVmac), was used to determine whether such a genetically restricted, T lymphocyte-mediated antiviral response could be generated in a primate. Vaccinia-SIVmac vaccination elicited an SIVmac Gag-specific, CD8+ CTL response in rhesus monkeys. These CTLs recognized a peptide fragment that spans residues 171 to 195 of the Gag protein. The rhesus monkey major histocompatibility complex (MHC) class I gene product restricting this CTL response was defined. Both the vaccinated and SIVmac-infected monkeys that shared this MHC class I gene product developed CTLs with the same Gag epitope specificity. These findings support the use of recombinant virus vaccines for the prevention of HIV infections in humans.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Shen, L -- Chen, Z W -- Miller, M D -- Stallard, V -- Mazzara, G P -- Panicali, D L -- Letvin, N L -- AI20729/AI/NIAID NIH HHS/ -- AI26507/AI/NIAID NIH HHS/ -- CA50139/CA/NCI NIH HHS/ -- etc. -- New York, N.Y. -- Science. 1991 Apr 19;252(5004):440-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Harvard Medical School, New England Regional Primate Research Center, Southborough, MA 01772.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1708168" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Animals
;
Antibodies, Viral/biosynthesis
;
Antigens, CD/analysis
;
Antigens, CD8
;
Antigens, Differentiation, T-Lymphocyte/analysis
;
Epitopes/chemistry/immunology
;
Gene Products, gag/chemistry/*immunology
;
Macaca mulatta
;
Molecular Sequence Data
;
Peptide Fragments/chemistry/immunology
;
Simian Acquired Immunodeficiency Syndrome/*immunology
;
Simian Immunodeficiency Virus/*immunology
;
T-Lymphocytes, Cytotoxic/*immunology
;
T-Lymphocytes, Helper-Inducer/immunology
;
Vaccines, Synthetic/*immunology
;
Viral Vaccines/*immunology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
Permalink
