ISSN:
1434-4475
Keywords:
Enzymic synthesis in biphasic systems
;
Papain-catalyzed peptide bond formation
;
Peptide synthesis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract Several model peptides have been synthesized enzymatically using papain as a catalyst in biphasic aqueous-organic systems. The effect of different cosolvents,pH, buffer concentration, and reaction time on the papaincatalyzed synthesis was examined. A comparison of the results obtained indicates that water-immiscible organic solvents provide higher yields than methanol in papain mediated peptide synthesis with carboxyl components in the carboxyl free form. Furthermore, it could be established that papaincatalyzed peptide synthesis can be considerably speeded up by employing acyl peptide esters instead of acyl peptides. The former should promote the rapid formation of the acyl-enzyme intermediate.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00905471
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