ISSN:
1573-4927
Keywords:
PHYLOGEOGRAPHY
;
MICROSATELLITE LOCI
;
MUSEUM SPECIMENS
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract A complete phylogeographic analysis of anyspecies requires sampling throughout its biogeographicalrange. In the case of the natterjack toad Bufocalamita in Britain, recent local extinctions haveleft substantial areas of its historical rangewithout extant populations. We therefore obtained tissuesamples of archived Bufo calamita from fourmuseums in the United Kingdom. A range of tissues(tongue, liver, skin, lung, and larval tail) was sampledfrom a total of 33 individual animals. DNA was extractedand eight polymorphic microsatellite loci were scored.One or more loci were amplified successfully from 27 individuals, and sufficient data wereobtained from regions with few or no survivingpopulations to supplement a phylogeographic analysisbased on extant populations.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1018776925261
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