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  • P.R. proteins  (1)
  • proline-rich  (1)
  • protoplasts  (1)
  • 1
    Electronic Resource
    Electronic Resource
    mRNAs newly synthesized by tobacco mesophyll protoplasts are wound-inducible (1990)
    Springer
    Plant molecular biology 15 (1990), S. 485-496 
    Details
    ISSN: 1573-5028
    Keywords: protoplasts ; P.R. proteins ; tobacco ; wounding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have used 2-dimensional (2D) non-equilibrium pH gradient gel electrophoresis (NEPHGE) of in vitro synthesized proteins and northern hybridization with labelled cDNAs coding for three pathogenesis related (P.R.) proteins, to analyze the shift in mRNA content induced by the isolation and culture of tobacco mesophyll protoplasts. The in vitro protein pattern of mRNAs from freshly isolated protoplasts is characterized by the absence of most leaf spots and the appearance of 19 new spots. After 6 hours of culture, the mRNAs coding for the P.R. proteins become detectable and after 12 hours the protoplasts contain an mRNA population almost typical of callus cells. The different steps involved in the isolation and culture of protoplasts were analysed. Cutting off the leaf and sterilization do not change the mRNA set. In contrast, the mechanical injury applied to the leaf in order to facilitate the penetration of the enzymatic mixture induces a modification of the mRNA content identical to that resulting from protoplast isolation. Wounding is the essential event inducing dedifferentiation. Varying the culture medium and conditions leads to only limited modifications of the mRNA pattern. These results are discussed on the basis of present knowledge of the reaction of the plant to wounding and we suggest that wound healing callus and in vitro callus correspond to the same differentiation state.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00019165
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Molecular characterization of the gene coding for GPRP, a class of proteins rich in glycine and proline interacting with membranes in Arabidopsis thaliana (1996)
    Springer
    Plant molecular biology 30 (1996), S. 625-636 
    Details
    ISSN: 1573-5028
    Keywords: Arabidopsis thaliana ; genomic cloning ; glycine-rich ; proline-rich ; protein-membrane interaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The gene coding for a new class of proteins rich in glycine and proline (GPRP) was cloned in Arabidopsis thaliana. In the protein sequence, five amino acids-glycine, proline, alanine, tyrosine and histidine-account for 79.4% of the total composition. The protein has two different glycine-rich domains interrupted by a hydrophobic segment having a high probability of helix formation. The protein synthesized in vitro interacts with microsomes possibly through the hydrophobic domain. The gene in Arabidopsis has two introns, one in the coding region and the other one in the 5′ non-coding region. The later one is 778 bp long. Homologous sequences are found in carrot, tomato and tobacco. GPRP mRNA is found in the different organs of the plant analyzed except in mature seeds and anthers, and mostly in epidermal and vascular tissues. Possible hypotheses about the function of GPRP are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00049336
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    Paper (German National Licenses)
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