ISSN:
1432-0983
Keywords:
Transformation
;
C. maltosa
;
P. guilliermondii
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Saccharomyces cerevisiae, Candida maltosa and Pichia guilliermondii have been transformed by the plasmid pYe(ARG4)411, which contains the S. cerevisiae ARG4 gene inserted into pBR322. In all transformants argininosuccinate lyase as well as β-lactamase were detected. The ARG+ phenotype of transformants is mitotically unstable. Closed circular pYe(ARG4)411, DNA was detected in transformant DNA preparations by hybridization to pBR322 DNA and by transformation of E. coli to ampicillin resistance.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00420313
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