ISSN:
1432-2145
Keywords:
Pistacia vera L
;
In vitro germination
;
Pollen
;
Osmotic effect
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The osmotic effect of Polyethylene glycol (PEG) has been shown to be sufficient to induce the germination of Pistacia vera L. pollen in liquid medium. The prehydration of the pollen in a saturated atmosphere for approximately 10 h was necessary to obtain maximum in vitro germination. Imbibition of the pollen in water resulted in the rapid leakage of solutes into the medium. These solutes consisted of approximately 50% carbohydrates, of which sucrose (0.65 μmol/mg), glucose (0.77 μmol/mg) and fructose (0.78 μmol/mg) were the major sugars; the remaining 50% comprised proteins with the following major molecular weights 63 kDa, 60 kDa, 59 kDa, 40 kDa, 36 kDa, 35.5 kDa, 31 kDa, other organic matter and minerals.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00190002
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