ISSN:
1612-1112
Keywords:
Reversed-phase chromatography
;
Column liquid chromatography
;
Isocratic elution
;
Optimization of stepwise elution
;
Chloro- and methylphenols
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary Equations describing multi-step gradient elution with a mobile phase of constant composition in each step were derived. These equations useful for calculating the retention volumes in both gradient HPLC and TLC were derived on the basis of the relationship between the isocratic capacity factor and the volume fraction of the organic modifier. The validity of the equations was experimentally verified in a LiChrosorbRP-18-water/methanol system for 11 methyl- and chlorobenzenes and phenols. A satisfactory agreement between the theoretical and experimental k′ values was found.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02311878
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