ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Ligand exchange
;
On-Line preconcentration
;
Opiate pentapeptide
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary In order to study the occurrence of the opiate pentapeptide β-[D-Ala2,4, Tyr5] CM-5-NH2 in the 10−9–10−5 M concentration range, we have developed a new method consisting of extraction of the peptide from its buffered aqueous medium onto a reversed phase cartridge of 50 μL void volume, followed by on-line injection onto a Cu(II) modified silica gel column. The acetonitrile-rich mobile phase allows detection of the natural fluorescence of the peptide at the picomole level.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02262093
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