ISSN:
1432-072X
Keywords:
Shigella lipopolysaccharide
;
O-antigen repeat units
;
Monoclonal antibodies
;
Immunoblots
;
Outer membranes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A procedure is described for the efficient transfer of fractionated bacterial lipopolysaccharide (LPS) from SDS-polyacrylamide gels to nitrocellulose filters, and its subsequent display by a peroxidase-linked antibody. The method is sensitive, and reveals and resolves high molecular weight LPS molecules having side chain lengths of up to and greater than 30 repeat units. It is also useful for the rapid analysis of LPS in bacterial outer membrane preparations.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00454926
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