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  • Nucleic acids  (1)
  • Temperature-gradient gel electrophoresis  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Thermostable variants of subtilisin selected by temperature-gradient gel electrophoresis (1996)
    Weinheim : Wiley-Blackwell
    Electrophoresis 17 (1996), S. 784-792 
    Details
    ISSN: 0173-0835
    Keywords: Thermal stability ; Random mutagenesis ; Screening ; Temperature-gradient gel electrophoresis ; Calcium binding site ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Region-specific random mutagenesis in the weak calcium binding site of subtilisin Carlsberg and subsequent screening for variants with enhanced heat stability revealed two variants, which showed significantly enhanced residual activity at 68°C, 0.1 mM CaCl2, pH 8.0. Preselected variants have been studied by temperature-gradient gel electrophoresis (TGGE) and were found to be stabilized due to different effects. Whereas the point mutation (Ser188Pro) mainly enhanced autoproteolytic stability of subtilisin, the double mutation (Ser188Pro; Ala194Glu) additionally increased the apparent Tm-value of the molecule for 2-3°C under a variety of conditions. It was possible to differentiate between the effects of autoproteolysis and structural unfolding to a certain degree by TGGE and to show the complex influence of changed calcium affinity on thermal stability for the double variant.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150170428
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  • 2
    Electronic Resource
    Electronic Resource
    Viroids: A Class of Subviral Pathogens (1980)
    Weinheim : Wiley-Blackwell
    Angewandte Chemie International Edition in English 19 (1980), S. 231-243 
    Details
    ISSN: 0570-0833
    Keywords: Viroids ; Pathogens ; Nucleic acids ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Infectious RNA molecules lacking a protein coat have recently been shown to be the cause of several diseases in higher plants. These molecules, termed viroids, have been characterized as circular chains composed of about 360 nucleotide residues. They therefore comprise a class of infectious pathogens even smaller than viruses or bacteriophages. As a result of work on viroids it is now possible for the first time to give a complete description of the structure of a eukaryotic pathogen. Viroids possess structural and dynamic properties which have not as yet been observed with other nucleic acids. This article summarizes and discusses the results of predominantly biochemical and physicochemical studies on viroids.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/anie.198002313
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