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  • Nucleic Acid Enzymology  (1)
  • Numerical Methods and Modeling  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    A projection method for shallow water equations (1998)
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 27 (1998), S. 81-95 
    Details
    ISSN: 0271-2091
    Keywords: shallow waters ; Navier-Stokes ; lagoons ; projections ; parallel computations ; Engineering ; Numerical Methods and Modeling
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The dynamics of shallow water has been studied and an algorithm for this dynamics has been developed. Results have been obtained with data of the Venice lagoon using a model made expressively by a semi-implicit method based on a finite element method in space. Comparison has been made between field data and the results of the simulation. Very good agreement is shown over a long period of simulation. © 1998 John Wiley & Sons, Ltd.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
  • 2
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    Probing the activity of diguanylate cyclases and c-di-GMP phosphodiesterases in real-time by CD spectroscopy (2013)
    Oxford University Press
    Details
    Publication Date: 2013-04-14
    Description: Bacteria react to adverse environmental stimuli by clustering into organized communities called biofilms. A remarkably sophisticated control system based on the dinucleotide 3'–5' cyclic diguanylic acid (c-di-GMP) is involved in deciding whether to form or abandon biofilms. The ability of c-di-GMP to form self-intercalated dimers is also thought to play a role in this complex regulation. A great advantage in the quest of elucidating the catalytic properties of the enzymes involved in c-di-GMP turnover (diguanylate cyclases and phosphodiesterases) would come from the availability of an experimental approach for in vitro quantification of c-di-GMP in real-time. Here, we show that c-di-GMP can be detected and quantified by circular dichroism (CD) spectroscopy in the low micromolar range. The method is based on the selective ability of manganese ions to induce formation of the intercalated dimer of the c-di-GMP dinucleotide in solution, which displays an intense sigmoidal CD spectrum in the near-ultraviolet region. This characteristic spectrum originates from the stacking interaction of the four mutually intercalated guanines, as it is absent in the other cyclic dinucleotide 3'–5' cyclic adenilic acid (c-di-AMP). Thus, near-ultraviolet CD can be used to effectively quantify in real-time the activity of diguanylate cyclases and phosphodiesterases in solution.
    Keywords: Nucleic Acid Enzymology
    Print ISSN: 0305-1048
    Electronic ISSN: 1362-4962
    Topics: Biology
    Published by Oxford University Press
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