ISSN:
1617-4623
Keywords:
Actin-related protein
;
Nuclear migration Cytoskeleton
;
Neurospora
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The ro-4 mutant of the filamentous fungus Neurospora crassa forms distinctive colonies in which hyphae grow into rope-like aggregates. This unusual morphology coincides with a defect in hyphal nuclear migration. The ro-4 gene was cloned from a cosmid library by complementation of the closely linked pab-2 gene. The deduced 380 amino acid protein is most similar to the vertebrate actin-related protein/centractin. The R04 protein is not essential for cell viability, and new strains created by inducing point mutations at the ro-4 locus have a phenotype which is very similar to that of the original mutant. This study provides genetic evidence that an actin-related protein plays a role in nuclear motility. Since nuclear motility is believed to be a microtubule-dependent process, the ro-4 gene product may function as a component of the dynactin complex which activates force generation by cytoplasmic dynein.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00290350
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