Publication Date:
2022-05-25
Description:
Author Posting. © The Author(s), 2017. This is the author's version of the work. It is posted here by permission of Taylor & Francis for personal use, not for redistribution. The definitive version was published in Nucleus 8 (2017): 222-237, doi:10.1080/19491034.2017.1285989.
Description:
In order to understand the chromatin changes underlying differential gene expression during
induced differentiation of human leukemic HL-60/S4 cells, we conducted RNA-Seq analysis
on quadruplicate cultures of undifferentiated, granulocytic- and macrophage-differentiated
cell forms. More than half of mapped genes exhibited altered transcript levels in the
differentiated cell forms. In general, more genes showed increased mRNA levels in the
granulocytic form and in the macrophage form, than showed decreased levels. The majority
of Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were significantly enriched in genes that exhibited differential transcript levels after either RA or TPA
treatment. Changes in transcript levels for groups of genes with characteristic protein
phenotypes, such as genes encoding cytoplasmic granular proteins, nuclear envelope and
cytoskeletal proteins, cell adhesion proteins, and proteins involved in the cell cycle and
apoptosis illustrate the profound differences among the various cell states. In addition to the
transcriptome analyses, companion karyotyping by M-FISH of undifferentiated HL-60/S4
cells revealed a plethora of chromosome alterations, compared to normal human cells. The
present mRNA profiling provides important information related to nuclear shape changes
(e.g., granulocyte lobulation), deformability of the nuclear envelope and linkage between the
nuclear envelope and cytoskeleton during induced myeloid chromatin differentiation.
Description:
DMW
thanks the Bay and Paul Foundations for support. ALO and DEO thank the College of
Pharmacy at UNE for their support. ALO and DEO are recipients of a 2015 UNE Mini-
Grant from the Vice President for Research and Scholarship. ALO and DEO thank the
German Cancer Research Center (Heidelberg) for the awards of Guest Scientist fellowships.
Description:
2018-02-02
Keywords:
Cell differentiation
;
mRNA levels
;
Acute Myeloid Leukema
;
Granulocyte
;
Macrophage
;
Nuclear envelope
;
Cytoskeleton
;
Cell division
;
Apoptosis
;
Cell attachment
;
Karyotype
Repository Name:
Woods Hole Open Access Server
Type:
Preprint
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