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  • 1
    Electronic Resource
    Electronic Resource
    Dominant negative mutations in the α-factor receptor, a G protein-coupled receptor encoded by the STE2 gene of the yeast Saccharomyces cerevisiae (1999)
    Springer
    Molecular genetics and genomics 261 (1999), S. 917-932 
    Details
    ISSN: 1617-4623
    Keywords: Key words G protein-coupled receptor ; STE2 ; α-Factor ; Yeast mating ; Dominant negative mutations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The α-mating pheromone receptor encoded by the STE2 gene of the yeast Saccharomyces cerevisiae is a G protein-coupled receptor (GPCR) that is homologous to the large family of GPCRs that mediate multiple types of signal transduction in mammals. We have screened libraries of mutant receptors to identify dominant negative alleles that are capable of interfering with the function of a co-expressed normal receptor. Two dominant negative alleles have been recovered in this manner. In addition, we find that previously isolated loss-of-function mutations in the α-factor receptor exhibit dominant negative effects. Detection of the dominant effects requires high-level expression of the mutant receptors but does not require a high ratio of mutant to normal receptors. Cellular levels of the normal receptors are not affected by co-expression of the dominant negative alleles. Expression of the mutant receptors does not interfere with constitutive signaling in a strain that lacks the G protein α subunit encoded by GPA1, indicating that interference with signaling occurs at the level of the receptor or the interacting G protein. Expression of increased levels of G protein subunits partially reverses the dominant negative effects. The dominant negative behavior of the mutant receptors is diminished by deletion of the SST2 gene, which encodes an RGS (Regulator of G protein Signaling) protein involved in desensitization of pheromone signaling. The most likely explanation for the dominant negative effects of the mutations appears to be the existence of an interaction between unactivated receptors and the trimeric G protein that titrates the G protein away from the normal receptors or renders the G protein insensitive to receptor activation. This interaction appears to be mediated by the SST2 gene product.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380051039
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    Long-range dynamic polarization potentials for ^{11} Be projectiles on ^{64} Zn (2015)
    American Physical Society (APS)
    Details
    Publication Date: 2015-07-30
    Description: Author(s): W. Y. So, K. S. Kim, K. S. Choi, and Myung-Ki Cheoun We investigate the effects of the long-range dynamic polarization (LRDP) potential, which consists of the Coulomb dipole excitation (CDE) potential and the long-range nuclear (LRN) potential, for the Be 11 projectile on Zn 64 . To study these effects, we perform a χ 2 analysis of an optical model includ… [Phys. Rev. C 92, 014627] Published Wed Jul 29, 2015
    Keywords: Nuclear Reactions
    Print ISSN: 0556-2813
    Electronic ISSN: 1089-490X
    Topics: Physics
    Published by American Physical Society (APS)
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    Long-range dynamic polarization potentials for Li11+Pb208 and He6+Pb208 systems (2014)
    American Physical Society (APS)
    Details
    Publication Date: 2014-11-21
    Description: Author(s): W. Y. So, K. S. Kim, K. S. Choi, and Myung-Ki Cheoun We investigate the effects of long-range dynamic polarization on elastic cross sections for heavy-ion collisions which comprise the Coulomb dipole excitation (CDE) potential and the long-range nuclear (LRN) potential. To study these effects, we perform a χ2 analysis for the elastic cross sections of... [Phys. Rev. C 90, 054615] Published Thu Nov 20, 2014
    Keywords: Nuclear Reactions
    Print ISSN: 0556-2813
    Electronic ISSN: 1089-490X
    Topics: Physics
    Published by American Physical Society (APS)
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