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  • Non-nodulating mutants  (1)
  • Siderophore utilization  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Siderophore production and utilization byRhizobium trifolii (1989)
    Springer
    BioMetals 2 (1989), S. 45-49 
    Details
    ISSN: 1572-8773
    Keywords: Siderophore production ; Siderophore utilization ; Rhizobium trifolii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary Several strains ofRhizobium trifolii were tested for their ability to synthesize and utilize phenolate or hydroxamate types of siderophores. None of the nodulating strains ofR. trifolii was able to produce detectable amounts of siderophores. Only the non-nodulating strainR. trifolii AR6 formed a phenolate siderophore, which stimulated the growth of the siderophore-negative mutant AR65. Other strains ofR. trifolii could not utilize iron from exogenously supplied Desferal, pseudobactin or citrate. The siderophore fromR. trifolii AR6 and 2,3-dihydroxybenzoic acid slightly stimulated the growth of someR. trifolii strains.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01116201
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Plasmid-mediated control of nodulation in Rhizobium trifolii (1979)
    Springer
    Archives of microbiology 123 (1979), S. 195-201 
    Details
    ISSN: 1432-072X
    Keywords: Rhizobium trifolii ; Nodulation ; Non-nodulating mutants ; Plasmids ; Molecular weight of plasmid ; Elimination of plasmid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The nodulation ability was effectively eliminated from different Rhizobium trifolii strains incubated at elevated temperature (Żurkowski and Lorkiewicz, 1978). Non-nodulating (Nod-) mutants were stable and no reversion of Nod- to Nod+ phenotype was observed. Strains R. trifolii 24 and T12 which showed a high percentage of elimination of nodulation ability were examined in detail. Two plasmids were detected in strain 24 using neutral and alkaline sucrose gradient centrifugation of plasmid preparations. Molecular weights of the plasmids pWZ1 and pWZ2 were 460 Mdal and 190 Mdal, respectively. Rhizobium lysates labeled with 3H-thymidine and ultracentrifuged in caesium chloride — ethidium bromide gradients demonstrated a 40% reduction of the plasmid DNA content in R. trifolii 24 Nod- mutants in comparison with the nodulating wild type strain 24. It was found further that non-nodulation of mutants 24 Nod- was due to the absence of plasmid pWZ2. Sucrose gradient data also demonstrated that strain T12 contained two plasmids with molecular weights corresponding to those of pWZ1 and pWZ2, respectively. In Nod- mutant clones derived from strain T12, pWZ2 plasmid was missing.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00446820
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    Paper (German National Licenses)
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