ISSN:
1432-072X
Keywords:
d-Fructose
;
New pathway
;
l-Sorbose
;
E. coli
;
K. pneumoniae
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Starting with a fruK (formerly fpk) mutant of Escherichia coli K12 lacking d-fructose-1-phosphate kinase (E.C. 2.7.1.3.), fructose positive derivatives were isolated after introduction of the cloned gene sorE from Klebsiella pneumoniae coding for an l-sorbose-1-phosphate reductase. The new pathway was shwon to proceed from d-fructose via d-fructose-1-phosphate and d-mannitol-1-phosphate to d-fructose 6-phosphate. It involves a transport system and enzymes encoded in the fru and the mtl operons from E. coli K12 as well as in the sor operon from K. pneumoniae respectively.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00423327
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