ISSN:
1573-5044
Keywords:
Nandina
;
berberine
;
culture medium
;
discoloration
;
plant morphogenesis
;
autotoxic substances
;
Nicotiana
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The release of endogenous substances is a general phenomenon of plant tissue cultures, with some substances having significant developmental effects on the releasing tissues. Their systematic study was initiated with Nandina tissue cultures, and a yellow compound that accumulated in the culture medium was identified as the alkaloid, berberine. The rate of its release was related to the supplies of auxin, cytokinin, and nutrient salts. Addition of berberine \ HCl to nutrient media did not inhibit Nandina tissues, but suppressed shoot formation in Nicotiana stem segments. Growth of Nandina and Nicotiana callus, as well as rooting of Nicotiana stem segments, was promoted by alkaloid addenda.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00041653
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