ISSN:
1572-8951
Keywords:
Trypanothione reductase
;
FAD
;
NADP
;
model-building
;
molecular dynamics
;
flavoprotein
;
trypanosomiasis
;
Chaga's disease
;
electron transfer mechanism
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract A three-dimensional structure is engineered for the Trypanosoma congolense trypanothione reductase (TpR) using the sequence homology with glutathione reductase (GR) and lipoamide dehydrogenase, molecular graphics, energy optimization and molecular dynamics techniques. The model was extended to include the complex with the coenzyme nicotinamide adenine dinucleotide phosphate (NADP). The TpR-NADP structure is compared with X-ray data from the glutathione reductase complex with the reduced NADP (NADPH). A model of TpR-NADP including the trypanothione substrate is presented, and an electron-transfer mechanism is proposed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00176807
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