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  • in vitro  (5)
  • NAA  (1)
  • Setaria italica  (1)
  • 1
    Electronic Resource
    Electronic Resource
    The role of nickel on somatic embryogenesis in Setaria italica L. in vitro (1998)
    Springer
    Euphytica 101 (1998), S. 319-324 
    Details
    ISSN: 1573-5060
    Keywords: nickel ; somatic embryogenesis ; tolerant cell lines ; in vitro ; Setaria italica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Nickel (0.13, 0.25, 0.5, 1.0 and 1.5 mg/l) increased the efficiency of somatic embryogenesis in leaf base and mesocotyl derived calli of Setaria italica. A lower concentration of nickel in the culture media promoted long-term maintenance of embryogenic calli that regenerated into plantlets. The plants obtained from embryogenic calli grown on Ni-containing medium showed tolerance to nickel. The growth of embryogenic callus was the maximum at 1.5 mg/l as compared to a lower or a higher concentration of Ni. Use of nickel may help in the induction of high frequency somatic embryogenesis in Setaria italica.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018386912104
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  • 2
    Electronic Resource
    Electronic Resource
    in Vitro Somatic Embryogenesis in Typhonium Trilobatum Schott. (1999)
    Springer
    Plant growth regulation 27 (1999), S. 195-199 
    Details
    ISSN: 1573-5087
    Keywords: growth regulator ; in vitro ; medicinal plant ; somatic embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract An in vitro protocol was developed for the production of plants via somatic embryogenesis in callus cultures derived from petiole and leaf explants of Typhonium trilobatum. Optimum callus formation was achieved on semisolid Murashige and Skoog's [9] medium supplemented with 0.25 mg L−1 kinetin and 3.0 mgL−1 1-naphthaleneacetic acid (NAA) after 6 weeks of culture. Somatic embryogenesis was achieved upon transferring the callus to a medium containing 1.0 mg Lminus 1 kinetin and 0.25 mg Lminus 1 NAA. In vitro tuberization was also achieved on medium containing 1/2 strength MS basal salts supplemented with 1.0 mg L−1 Kinetin and 0.1 mg L−1 NAA. Embryo maturation and germination was achieved on the MS basal salts supplemented with 0.01 mg L−1 NAA and 2% (w/v) sucrose. Some thousands somatic embryo derived plantlets were hardened in the greenhouse and eventually planted in the open field.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006278716645
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  • 3
    Electronic Resource
    Electronic Resource
    Plant regeneration from callus cultures of Psoralea corylifolia Linn (1997)
    Springer
    Plant growth regulation 22 (1997), S. 13-17 
    Details
    ISSN: 1573-5087
    Keywords: BA ; in vitro ; medicinal plant ; NAA ; regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Plantlet regeneration via organogenesis was achieved in callus cultures derived form mature leaves, stems and leaves, petioles and roots of young seedling of Psoralea corylifolia on Murashige and Skoog medium supplemented with 2.5–3.0 mg L-1 BA, 1.0 mg L-1 NAA and 3% (w/v) sucrose. The rate of shoot bud regeneration was positively correlated with the concentration of hormones in the nutrient media. Shoot buds regenerated more readily from juvenile explants (seedling source) as compared to the mature explants. Addition of adenine sulphate (5 mg L-1) to the culture medium increased the growth of shoot buds. Optimum responses were obtained in hypocotyl and leaf explants using NAA in combination with BA, the highest rate of shoot bud regeneration being in hypocotyl explants. Rooting was readily achieved on the differentiated shoots on MS basal media without growth regulators. Regenerated plantlets were successfully established in the greenhouse.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005869404510
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  • 4
    Electronic Resource
    Electronic Resource
    In vitro rooting of Psoralea corylifolia Linn: Peroxidase activity as a marker (2000)
    Springer
    Plant growth regulation 30 (2000), S. 215-219 
    Details
    ISSN: 1573-5087
    Keywords: growth regulator ; in vitro ; medicinal plant ; peroxidase activity ; rooting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Induction of rooting in microshoots of Psoraleacorylifolia was achieved within 6–8 days of cultureon half-strength basal Murashige and Skoog's(1962) medium supplemented with 0.005–0.01 mg/lindole-3-acetic acid (IAA) and 2% (w/v) sucrose. Rooting was drastically reduced and friable callusformed at the cut end of the microshoots when themedium was supplemented with a higher concentration ofauxin. Rooting was totally inhibited when themicroshoots were cultured in vitro undercontinuous light. However, the maximum percentage ofmicroshoots rooted when incubated in continuous lightfor 4 weeks before transfer to the rooting media.Peroxidase activity increased considerably duringroot induction indicating a key role of peroxidase inrooting of microshoots of Psoralea corylifolia invitro.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006336819887
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  • 5
    Electronic Resource
    Electronic Resource
    Somatic embryogenesis and plant regeneration from callus culture of Acacia catechu-a multipurpose leguminous tree (1995)
    Springer
    Plant cell, tissue and organ culture 42 (1995), S. 283-285 
    Details
    ISSN: 1573-5044
    Keywords: in vitro ; L-proline ; legume ; tropical tree
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Plant regeneration via somatic embryogenesis was achieved from callus derived from immature cotyledons of Acacia catechu Willd. on Woody Plant Medium (WPM) supplemented with 13.9 μM kinetin and 2.7 μM 1-naphthaleneacetic acid. The addition of 0.9–3.5 mM L-proline to the medium influenced development of somatic embryos and also promoted secondary somatic embryogenesis. The light-green somatic embryos germinated on half-strength MS medium supplemented with 2% (w/v) sucrose. Somatic embryos germinated into plantlets that were acclimatized in the greenhouse and subsequently transferred to the field.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00030000
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