Publication Date:
2008-12-20
Description:
Selection pressure exerted by insects and microorganisms shapes the diversity of plant secondary metabolites. We identified a metabolic pathway for glucosinolates, known insect deterrents, that differs from the pathway activated by chewing insects. This pathway is active in living plant cells, may contribute to glucosinolate turnover, and has been recruited for broad-spectrum antifungal defense responses. The Arabidopsis CYP81F2 gene encodes a P450 monooxygenase that is essential for the pathogen-induced accumulation of 4-methoxyindol-3-ylmethylglucosinolate, which in turn is activated by the atypical PEN2 myrosinase (a type of beta-thioglucoside glucohydrolase) for antifungal defense. We propose that reiterated enzymatic cycles, controlling the generation of toxic molecules and their detoxification, enable the recruitment of glucosinolates in defense responses.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Bednarek, Pawel -- Pislewska-Bednarek, Mariola -- Svatos, Ales -- Schneider, Bernd -- Doubsky, Jan -- Mansurova, Madina -- Humphry, Matt -- Consonni, Chiara -- Panstruga, Ralph -- Sanchez-Vallet, Andrea -- Molina, Antonio -- Schulze-Lefert, Paul -- New York, N.Y. -- Science. 2009 Jan 2;323(5910):101-6. doi: 10.1126/science.1163732. Epub 2008 Dec 18.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Plant Microbe Interactions, Max Planck Institut fur Zuchtungsforschung, Carl-von-Linne-Weg 10, D-50829 Koln, Germany. bednarek@mpiz-koeln.mpg.de〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19095900" target="_blank"〉PubMed〈/a〉
Keywords:
Arabidopsis/genetics/immunology/*metabolism/*microbiology
;
Arabidopsis Proteins/genetics/*metabolism
;
Ascomycota/growth & development/*pathogenicity
;
Cysteine/metabolism
;
Cytochrome P-450 Enzyme System/genetics/metabolism
;
Genes, Plant
;
Glucosinolates/*metabolism
;
Glycoside Hydrolases/genetics/metabolism
;
Indoles/*metabolism
;
Metabolic Networks and Pathways
;
Mutation
;
N-Glycosyl Hydrolases/genetics/*metabolism
;
Plant Diseases/immunology/*microbiology
;
Thiazoles/metabolism
;
Thiones/metabolism
;
Tryptophan/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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