Publication Date:
1986-04-25
Description:
A combination of genetics, biochemistry, and biophysics was used to show that calmodulin is involved in the regulation of an ion channel. Calmodulin restored the Ca2+-dependent K+ current in pantophobiac, a mutant in Paramecium that lacks this current. The restoration of the current occurred within 2 hours after the injection of 1 picogram of wild-type calmodulin into the mutant. The current remained for approximately 30 hours before the mutant phenotype returned. The injection of calmodulin isolated from pantophobiac had no effect. These results imply that calmodulin is required for the function or regulation of the Ca2+-dependent K+ current in Paramecium.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hinrichsen, R D -- Burgess-Cassler, A -- Soltvedt, B C -- Hennessey, T -- Kung, C -- 1 F 32 NS 07502-01/NS/NINDS NIH HHS/ -- GM 22714/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1986 Apr 25;232(4749):503-6.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2421410" target="_blank"〉PubMed〈/a〉
Keywords:
Calcium/physiology
;
Calmodulin/*pharmacology
;
Dictyostelium/metabolism
;
Ion Channels/*drug effects/physiology
;
Mutation
;
Paramecium/drug effects/genetics/*metabolism
;
Potassium/*metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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