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  • Mutant streptomycin phosphotransferase  (1)
  • gene dosage  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Effects of gene dosage and sequence modification on the frequency and timing of transposition of the maize element Activator (Ac) in tobacco (1993)
    Springer
    Plant molecular biology 21 (1993), S. 157-170 
    Details
    ISSN: 1573-5028
    Keywords: transposable element ; Activator ; gene dosage ; maize ; tobacco
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of Ac copy number on the frequency and timing of germinal transposition in tobacco was investigated using the streptomycin phosphotransferase gene (SPT) as an excision marker. The activity of one and two copies of the element was compared by selecting heterozygous and homozygous progeny of transformants carrying single SPT::Ac inserts. It was observed that increasing gene copy not only increases the transposition frequency, but also occasionally alters the timing of transposition such that earlier events are obtained. The result is that some homozygous plants generate multiple streptomycin resistant progeny carrying the same transposed Ac (trAc) element. We have also investigated the effect of modification of the sequence in the region around 82 bp downstream of the polyadenylation site and 177 bp from the 3′ end of the element on germinal excision frequencies. Alteration of three bases to create a BglII site at this location caused a minor decrease in germinal excision events, but insertion of four bases to create a Cla I site caused a 10-fold decrease in the transposition activity of the Ac element.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039626
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  • 2
    Electronic Resource
    Electronic Resource
    Improved expression of streptomycin resistance in plants due to a deletion in the streptomycin phosphotransferase coding sequence (1988)
    Springer
    Molecular genetics and genomics 214 (1988), S. 456-459 
    Details
    ISSN: 1617-4623
    Keywords: Chimeric gene ; Mutant streptomycin phosphotransferase ; Non-lethal screen ; Streptomycin resistance ; Transgenic Nicotiana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Previous studies have shown that a chimeric streptomycin phosphotransferase (SPT) gene can function as a dominant marker for plant cell transformation. The SPT marker previously described by Jones and co-workers has a limited value since it conferred a useful level of resistance only to a fraction (10%) of Nicotiana plumbaginifolia transgenic lines. Expression of resistance was species specific: no such resistant transformants were found in N. tabacum. In this paper we describe an improved SPT construct that utilizes a mutant Tn5 SPT gene. The mutant gene, SPT *, encodes a protein with a two amino acid deletion close to its COOH-terminus. In N. tabacum cell culture the efficiency of transformation with the improved streptomycin resistance marker was comparable to kanamycin resistance. When the chimeric SPT * gene was introduced linked to a kanamycin resistance gene, streptomycin resistance was expressed in most of the transgenic N. tabacum lines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330480
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    Paper (German National Licenses)
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