ISSN:
1432-0983
Keywords:
Yeast
;
Mutant
;
Uncoupler
;
Resistance
;
Permeability
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary One mutant resistant to carbonylcyanide m-chlorophenylhydrazone (CCCP), an uncoupler of oxidative phosphorylation, was isolated in Saccharomyces cerevisiae. Genetic analysis showed that a single nuclear gene is responsible for increased resistance; this gene was dominant. The mutant showed cross-resistance or collateral sensitivity to several chemically-unrelated inhibitors (cycloheximide, dinitrophenol, tributhyltin chloride, chloramphenicol). The resistance of the mutant is related to a decreased uptake of CCCP which is not expressed in glucose-starved cells. It was shown that glucose induced a CCCP efflux which was more efficient in the mutant than in the wild-type cells. This effect was correlated to a greater acidification of the internal pH by glucose addition in the mutant cells. It was proposed that resistance was not due to a change of permeability of the plasmic membrane itself but to the change of internal pH which determines the extent of accumulation of weak acids or bases.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00410437
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