ISSN:
1040-452X
Keywords:
Mouse Preimplantation Embryo
;
Gene Expression
;
Growth Factor
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
Notes:
Although mouse preimplantation embryos express transcripts for the insulin-like growth factor-I receptor (IGF-IR), IGF-I transcripts were not detected by reverse transcription-PCR (RT-PCR) at any stage of preimplantation development in a previous study (Rappolee et al., Genes Dev 6:939-952, 1992). We report that IGF-I transcripts are detected in the preimplantation embryo by RT-PCR and describe the temporal pattern of expression of this transcript from the oocyte to blastocyst stages; the level declines from the oocyte to 8-cell embryo and then increases from the 8-cell to blastocyst stages. An explanation is offered to account for the differences in detecting the IGF-I transcript, and the results are discussed in the context of an autocrine/paracrine IGF-I circuit in the preimplantation mouse embryo. © 1994 Wiley-Liss, Inc.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/mrd.1080370104
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