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  • Monoclonal antibodies  (1)
  • Phototransduction  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 244 (1986), S. 315-319 
    ISSN: 1432-0878
    Keywords: Calmodulin ; Actin ; Photoreceptor membrane ; Microvilli ; Cytoskeleton ; Phototransduction ; Calcium ions ; Arthropods ; Squid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A monoclonal antibody against pea-leaf calmodulin was used to localise this calcium-binding protein on frozen sections of compound eyes of several arthropod species and on nitrocellulose replicas of electrophoretically separated peptides of isolated photoreceptor membrane from crayfish, fly, and squid. We report the presence of immunochemically detectable amounts of calmodulin specifically associated with the photoreceptor microvilli of rhabdomeral photoreceptors. A weak immunofluorescent signal was also observed in the cytoplasm of retinula cells. The presence of calmodulin in rhabdomeral microvilli is discussed in view of its possible implication in phototransduction and/or involvement in cytoskeletal structures associated with photoreceptor membranes in invertebrates.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1615-6102
    Keywords: Basal body ; Cytoskeleton ; Microtubule ; Immunoflu orescence ; Monoclonal antibodies ; Pteridium ; Spermatozoid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Detergent extracted spermatozoids of the fernPteridium aquilinum were used as mixed antigen preparations for raising monoclonal antibodies in order to obtain reagents for detecting as yet uncharacterized components of the plant cytoskeleton. Selected antibodies were studied by immunofluorescence microscopy of developing spermatids and mature spermatozoids. Some reacted directly with fixed cells, others required permeabilization treatments with cold methanol or Triton X-100. AntibodiesPas2D9 andPas6D7 bind to glycoprotein antigenic determinants that are exposed on the surface of the plasma membrane. Several antibodies interact with cytoskeletal components.Pas1D3,Pas5D8 andPas5F4 bind to the cytoskeleton of permeabilized cells including the flagella. Three react specifically with the flagellar band or associated components:Pas2G6 reacts with the whole flagellar band but shows a prominent binding to basal bodies,Pas5E2 binds exclusively to basal bodies, andPas5E7 detects mitochondria associated with the flagellar band. Cross-reactions to wheat root tip cells at different stages of the cell cycle are described inMarc andGunning (1988).
    Type of Medium: Electronic Resource
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