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  • Monascus pigments  (2)
  • Nutrition  (2)
  • Rapamycin biosynthesis  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Leucine interference in the production of water-soluble red Monascus pigments (1994)
    Springer
    Archives of microbiology 162 (1994), S. 114-119 
    Details
    ISSN: 1432-072X
    Keywords: Pigments ; Monascus pigments ; Leucine ; Amino acids ; Secondary metabolism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The formation of soluble Monascus red pigments is strongly positively and negatively regulated by different amino acids. Leucine, valine, lysine, and methionine had strong negative effects on pigment formation. Leucine supported poor pigment formation when used as sole nitrogen source in fermentations, yet it neither repressed pigment synthase(s) nor inhibited its action. The new pigments derived from the hydrophobic leucine were more hydrophilic than the conventional red pigments (lacking an amino acid side-chain) and were extracellularly produced. Therefore, the low level of red pigments produced when leucine was the nitrogen source was not due to feed-back regulation by cell-bound leucine pigments. The negative effect of leucine was caused by enhanced decay of pigment synthase(s). The enhanced decay was not due simply to de novo synthesis of a leucine-induced protease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00264383
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  • 2
    Electronic Resource
    Electronic Resource
    Leucine interference in the production of water-soluble red Monascus pigments (1994)
    Springer
    Archives of microbiology 162 (1994), S. 114-119 
    Details
    ISSN: 1432-072X
    Keywords: Key words     Pigments ; Monascus pigments ; Leucine ; Amino acids ; Secondary metabolism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract      The formation of soluble Monascus red pigments is strongly positively and negatively regulated by different amino acids. Leucine, valine, lysine, and methionine had strong negative effects on pigment formation. Leucine supported poor pigment formation when used as sole nitrogen source in fermentations, yet it neither repressed pigment synthase(s) nor inhibited its action. The new pigments derived from the hydrophobic leucine were more hydrophilic than the conventional red pigments (lacking an amino acid side-chain) and were extracellularly produced. Therefore, the low level of red pigments produced when leucine was the nitrogen source was not due to feedback regulation by cell-bound leucine pigments. The negative effect of leucine was caused by enhanced decay of pigment synthase(s). The enhanced decay was not due simply to de novo synthesis of a leucine-induced protease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050111
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Phosphate, ammonium, magnesium and iron nutrition ofStreptomyces hygroscopicus with respect to rapamycin biosynthesis (1995)
    Springer
    Journal of industrial microbiology and biotechnology 14 (1995), S. 424-427 
    Details
    ISSN: 1476-5535
    Keywords: Phosphate ; Ammonium ; Magnesium ; Iron ; Nutrition ; Rapamycin biosynthesis ; Streptomyces hygroscopicus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Phosphate, ammonium and magnesium salts interfered with rapamycin production byStreptomyces hygroscopicus at concentrations optimal for growth. These observations point to the existence of phosphorus, magnesium and nitrogen-negative regulation mechanisms for rapamycin biosynthesis. On the other hand, Fe2+ stimulated rapamycin production at concentrations greater than that required for growth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01569962
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  • 4
    Electronic Resource
    Electronic Resource
    Carbon source nutrition of rapamycin biosynthesis inStreptomyces hygroscopicus (1995)
    Springer
    Journal of industrial microbiology and biotechnology 14 (1995), S. 436-439 
    Details
    ISSN: 1476-5535
    Keywords: Rapamycin biosynthesis ; Carbon sources ; Nutrition ; Streptomyces hygroscopicus ; Immunosuppressants ; Antibiotics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Chemically-defined media were developed for rapamycin production byStreptomyces hygroscopicus. Thirty-five carbon sources were tested for their effect on production. Eight failed to support growth and seven appeared to repress or inhibit rapamycin formation. The best combination of two carbon sources were 2% fructose and 0.5% mannose. Acetate and propionate, which are known to contribute most of the carbon atoms of the lactone ring, were unsatisfactory for growth and/or rapamycin production.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01573954
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