Publication Date:
1996-05-10
Description:
A cofactor for HIV-1 (human immunodeficiency virus-type 1) fusion and entry was identified with the use of a novel functional complementary DNA (cDNA) cloning strategy. This protein, designated "fusin," is a putative G protein-coupled receptor with seven transmembrane segments. Recombinant fusin enabled CD4-expressing nonhuman cell types to support HIV-1 Env-mediated cell fusion and HIV-1 infection. Antibodies to fusin blocked cell fusion and infection with normal CD4-positive human target cells. Fusin messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types. Fusin acted preferentially for T cell line-tropic isolates, in comparison to its activity with macrophagetropic HIV-1 isolates.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Feng, Y -- Broder, C C -- Kennedy, P E -- Berger, E A -- New York, N.Y. -- Science. 1996 May 10;272(5263):872-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases (NIAID), NIH, Bethesda, MD 20892, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8629022" target="_blank"〉PubMed〈/a〉
Keywords:
3T3 Cells
;
Amino Acid Sequence
;
Animals
;
Antigens, CD4/*physiology
;
CD4-Positive T-Lymphocytes/virology
;
Cell Line
;
Cell Membrane/virology
;
Chemokines/physiology
;
*Cloning, Molecular
;
DNA, Complementary/genetics
;
Disease Models, Animal
;
GTP-Binding Proteins/metabolism
;
Giant Cells
;
HIV Envelope Protein gp120/physiology
;
HIV-1/*pathogenicity/physiology
;
HeLa Cells
;
Humans
;
Leukocytes, Mononuclear/virology
;
Macrophages/virology
;
*Membrane Fusion
;
Membrane Proteins/genetics/*physiology
;
Mice
;
Molecular Sequence Data
;
RNA, Messenger/metabolism
;
Receptors, CXCR4
;
Recombinant Proteins
;
Transfection
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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