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The case of the elusive tm values in HPLC (1989)

Daignault, L. G. ; Jackman, D. C. ; Rillema, D. P.

Springer

Chromatographia 27 (1989), S. 156-158

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ISSN: 1612-1112

Keywords: Column liquid chromatography ; Mobile phase retention time ; Capacity factor ; Negative capacity factor

Source: Springer Online Journal Archives 1860-2000

Topics: Chemistry and Pharmacology

Notes: Summary Numerous ideas and procedures have been suggested in the literature for the determination of tm, the retention time of a non-retained species, in high-performance liquid chromatography. In some cases chromatograms have been obtained showing sample components eluting prior to the assumed non-retained species. This phenomenon results in apparent negative capacity factors for the species in question. We have proposed a method employing small inorganic anions which results in a limiting value for tm and eliminates apparent negative capacity factors.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF02265868

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