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  • 1
    Electronic Resource
    Electronic Resource
    Time course and cellular site of mitotic activity in the exocrine pancreas of the rat during sustained hormone stimulation (1987)
    Springer
    Cell & tissue research 247 (1987), S. 385-391 
    Details
    ISSN: 1432-0878
    Keywords: Exocrine pancreas ; Caerulein ; DNA synthesis ; Mitotic activity ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Previous studies from our laboratory indicate that the adaptive response of the exocrine pancreas of the rat to prolonged stimulation with optimal doses of caerulein (0.25 μg × kg-1 × h-1) follows a characteristic time course in which each step in the secretory pathway is activated. The immediate response is the depletion of zymogen-granule stores followed by coordinate and anticoordinate changes in individual rates of (pro-)enzyme synthesis after a lag period of 2 h. The sum of such changes leads to an increase in total rate of protein synthesis by 3 h which is combined with acceleration of intracellular transport packaging and granule discharge. In the present study the time course of DNA synthesis and the labeling index of five populations of pancreatic cells have been analyzed after caerulein stimulation for periods ranging from 6 to 72 h, using in vivo labeling with 1 μCi/g 3H-thymidine 1 h prior to sacrifice of the animals. DNA synthesis did not change during the initial 18 h in spite of persistent stimulation indicated by a 80% reduction of enzyme content. Following this lag period a sharp rise in DNA synthesis 20- to 25-fold above control levels was observed, which decreased by 48 h to reach control levels by 72 h. Increase in DNA synthesis was most pronounced in animals with lowest enzyme content in the pancreas. From the five cell populations studied by autoradiography interlobular duct cells and islet cells had no significant increase in labeling index at any time of stimulation. Acinar cells, intralobular duct cells and interstitial cells showed a marked increase in labeling index after a latent period of 18 h with peak values at 36 h 30 to 50 times higher in intralobular duct and acinar cells, respectively, and 4 times higher in interstitial cells. The increased labeling indices in all three cell populations reverted to lower values at 48 h and reached control values by 72 h. The data indicate a phasic and limited growth response of the rat exocrine pancreas to persistent stimulation with acinar cells as the major contributing cell population.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00218320
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  • 2
    Electronic Resource
    Electronic Resource
    Stimulation of pancreatic secretory process in the rat by low-molecular weight proteinase inhibitor (1990)
    Springer
    Cell & tissue research 262 (1990), S. 143-148 
    Details
    ISSN: 1432-0878
    Keywords: Exocrine pancreas ; Proteinase inhibitor-DNA synthesis ; Mitotic activity ; Rat(Wistar)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Previous studies with rats have shown that a single oral dose of the proteinase inhibitor Camostate (FOY-305) induces release of cholecystokinin (CCK) into the circulation, which lasts for 3 to 6h. This transient endogenous release of hormone results in a depletion of pancreatic enzyme stores within 1 h and an increase in total rate of protein synthesis, which peaks at 6 to 9 h. At the level of individual enzyme biosynthesis a transient decrease in amylase and an increase in trypsinogen and chymotrypsinogen is observed. In the present study the time course of DNA synthesis and the labeling index of 5 populations of pancreatic cells have been analysed following a single oral dose of 50 or 100 mg/kg proteinase inhibitor, using in vivo labeling with 12 μCi/g body weight 3H-thymidine 1 h prior to sacrifice of the animals. DNA synthesis did not change during the initial 12 h following inhibitor feeding and then showed a phasic increase with a peak (20-fold) at 24h and intermediate increases (4- to 5-fold) at 18 and 36 h, respectively. From the 5 pancreatic cell populations studied by autoradiography the labeling indices of interlobular duct cells and islet cells did not change over the entire observation period. Acinar cells, intralobular duct cells and interstitial cells showed a marked increase in labeling index with peak values at 24h, which were 20-fold in acinar cells and 5.5- and 8.5-fold in intralobular duct cells and interstitial cells, respectively. The data demonstrate a significant growth response of pancreatic acinar tissue after a single episode of endogenous CCK-release, which is similar in extent, time course and cellular source as previously demonstrated during persistent stimulation of the pancreas by prolonged infusion of the CCK-analogue caerulein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00327755
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