Publication Date:
1987-04-10
Description:
A cycloheximide-sensitive protein responsive to adenosine 3',5'-monophosphate has been postulated to participate in the regulation of cholesterol side-chain cleavage activity in steroidogenic tissues. Such a steroidogenesis activator polypeptide (SAP) had been isolated from rat adrenocortical tissue and partially characterized. Now a polypeptide with comparable chromatographic behavior and biological activity has been purified from the rat H-540 Leydig cell tumor in quantities sufficient for amino acid sequencing. The activator contains 30 amino acid residues and has a molecular weight of 3215. The synthetic construct based on this sequence is virtually equipotent with native H-540 tumor SAP in an adrenal mitochondrial cholesterol side-chain cleavage assay. Hormonal regulation of the intracellular concentration of this activator may control the rate of cholesterol metabolism in steroidogenic organs.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Pedersen, R C -- Brownie, A C -- AM18141/AM/NIADDK NIH HHS/ -- HD00613/HD/NICHD NIH HHS/ -- HD19309/HD/NICHD NIH HHS/ -- New York, N.Y. -- Science. 1987 Apr 10;236(4798):188-90.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3563495" target="_blank"〉PubMed〈/a〉
Keywords:
Adrenal Cortex/analysis
;
Amino Acid Sequence
;
Animals
;
Cholesterol/metabolism
;
Cholesterol Side-Chain Cleavage Enzyme/*metabolism
;
Chromatography, High Pressure Liquid
;
*Heat-Shock Proteins
;
Leydig Cell Tumor/*analysis
;
Male
;
Mitochondria/enzymology
;
*Molecular Chaperones
;
Oxidoreductases/*metabolism
;
Peptide Fragments/analysis
;
Proteins/*analysis
;
Rats
;
Steroids/*biosynthesis
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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