Publication Date:
2009-06-27
Description:
Communication between organelles is an important feature of all eukaryotic cells. To uncover components involved in mitochondria/endoplasmic reticulum (ER) junctions, we screened for mutants that could be complemented by a synthetic protein designed to artificially tether the two organelles. We identified the Mmm1/Mdm10/Mdm12/Mdm34 complex as a molecular tether between ER and mitochondria. The tethering complex was composed of proteins resident of both ER and mitochondria. With the use of genome-wide mapping of genetic interactions, we showed that the components of the tethering complex were functionally connected to phospholipid biosynthesis and calcium-signaling genes. In mutant cells, phospholipid biosynthesis was impaired. The tethering complex localized to discrete foci, suggesting that discrete sites of close apposition between ER and mitochondria facilitate interorganelle calcium and phospholipid exchange.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2933203/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2933203/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kornmann, Benoit -- Currie, Erin -- Collins, Sean R -- Schuldiner, Maya -- Nunnari, Jodi -- Weissman, Jonathan S -- Walter, Peter -- R01 GM032384/GM/NIGMS NIH HHS/ -- R01 GM032384-27/GM/NIGMS NIH HHS/ -- R01 GM062942/GM/NIGMS NIH HHS/ -- Howard Hughes Medical Institute/ -- New York, N.Y. -- Science. 2009 Jul 24;325(5939):477-81. doi: 10.1126/science.1175088. Epub 2009 Jun 25.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, CA 94158, USA. benoit.kornmann@ucsf.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19556461" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Calcium Signaling/genetics
;
Endoplasmic Reticulum/*physiology
;
Membrane Proteins/*metabolism
;
Mice
;
Mitochondria/*physiology
;
Mitochondrial Proteins/*metabolism
;
Phospholipids/biosynthesis
;
Recombinant Fusion Proteins/genetics/metabolism
;
Saccharomyces cerevisiae
;
Saccharomyces cerevisiae Proteins/*metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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