Publikationsdatum:
2014-10-11
Beschreibung:
During animal cell division, the cleavage furrow is positioned by microtubules that signal to the actin cortex at the cell midplane. We developed a cell-free system to recapitulate cytokinesis signaling using cytoplasmic extract from Xenopus eggs. Microtubules grew out as asters from artificial centrosomes and met to organize antiparallel overlap zones. These zones blocked the interpenetration of neighboring asters and recruited cytokinesis midzone proteins, including the chromosomal passenger complex (CPC) and centralspindlin. The CPC was transported to overlap zones, which required two motor proteins, Kif4A and a Kif20A paralog. Using supported lipid bilayers to mimic the plasma membrane, we observed the recruitment of cleavage furrow markers, including an active RhoA reporter, at microtubule overlaps. This system opens further approaches to understanding the biophysics of cytokinesis signaling.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4281018/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4281018/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Nguyen, Phuong A -- Groen, Aaron C -- Loose, Martin -- Ishihara, Keisuke -- Wuhr, Martin -- Field, Christine M -- Mitchison, Timothy J -- GM103785/GM/NIGMS NIH HHS/ -- GM39565/GM/NIGMS NIH HHS/ -- R01 GM039565/GM/NIGMS NIH HHS/ -- R01 GM103785/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2014 Oct 10;346(6206):244-7. doi: 10.1126/science.1256773.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA. Marine Biological Laboratory, Woods Hole, MA 02543, USA. ; Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA. ; Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA. Marine Biological Laboratory, Woods Hole, MA 02543, USA. Christine_Field@hms.harvard.edu.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/25301629" target="_blank"〉PubMed〈/a〉
Schlagwort(e):
Animals
;
Cell Membrane/chemistry/*physiology
;
*Cell-Free System
;
Centrosome/physiology
;
*Cytokinesis
;
DNA-Binding Proteins/genetics/metabolism
;
Guanosine Triphosphate/metabolism
;
Kinesin/genetics/metabolism
;
Lipid Bilayers
;
Microtubules/physiology
;
Models, Biological
;
Nuclear Proteins/genetics/metabolism
;
*Signal Transduction
;
Xenopus laevis
;
rhoA GTP-Binding Protein/metabolism
Print ISSN:
0036-8075
Digitale ISSN:
1095-9203
Thema:
Biologie
,
Chemie und Pharmazie
,
Informatik
,
Medizin
,
Allgemeine Naturwissenschaft
,
Physik
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